thermanaerobacter_thermophila_medium

CultureMech:000305 archaea COMPLEX LIQUID
Original name: THERMANAEROBACTER THERMOPHILA MEDIUM

Overview

External ontology
mediadive.medium:J959 (source link unavailable) — THERMANAEROBACTER THERMOPHILA MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=959

Composition

flowchart LR
    CultureMech_000305["thermanaerobacter_thermophila_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_000305 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000305 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000305 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_000305 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000305 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000305 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KCl["KCl"]
    CultureMech_000305 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000305 --> ing7_Yeast_extract
    ing8_Resazurin["Resazurin"]
    CultureMech_000305 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_NaHCO3["NaHCO3"]
    CultureMech_000305 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Glucose["Glucose"]
    CultureMech_000305 --> ing10_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing10_Glucose -.-> CHEBI_17234
    ing11_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000305 --> ing11_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing11_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000305 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.297324 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.297324 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.297324 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
0.99108 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.49554 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.148662 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 KCl
Cl.K 74.551 g/mol
0.099108 G_PER_L CHEBI:32588
potassium chloride
8 Yeast extract 0.49554 G_PER_L
9 Resazurin
C12H7NO4 229.191 g/mol
0.00099108 G_PER_L CHEBI:8806
Resazurin
10 NaHCO3
CHO3.Na 84.006 g/mol
25 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
12 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
20 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
8 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Mix components thoroughly, adjust pH to 7.2 with KOH, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 935 ml of the medium) from anaerobic stocks (autoclaved or *filter-sterilized):

Solutions

Trace element solution

Dose: 1 G_PER_L

See mediadive_6187_Trace_element_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:18.137610Z mediadive-import Imported from MediaDive Source: JCM, ID: J959
2026-03-09T08:05:15.211683+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:18:54.332549Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000305
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T17:06:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.297247+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMANAEROBACTER THERMOPHILA MEDIUM" to "thermanaerobacter_thermophila_medium" for consistent matching
2026-05-16T09:02:57.830256+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; category->lowercase=1
2026-06-05T07:40:15.131375+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.394167+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.