s_w_amp

CultureMech:000307 bacterial COMPLEX BIPHASIC
Original name: S/W + AMP

Overview

External ontology
mediadive.medium:C100 (source link unavailable) — S/W + AMP
Applications
  • Microbial cultivation
pH range
{'min': 7.0, 'max': 8.0}
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_SW_AMP.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_SW_AMP.pdf on 2026-08-25.

Composition

flowchart LR
    CultureMech_000307["s_w_amp"]
    ing0_Ammonium_magnesium_phosphate["Ammonium magnesium phosphate"]
    CultureMech_000307 --> ing0_Ammonium_magnesium_phosphate
    CHEBI_149425["CHEBI:149425"]
    ing0_Ammonium_magnesium_phosphate -.-> CHEBI_149425
    ing1_Air_dried_sieved_calcareous_soil["Air-dried sieved calcareous soil"]
    CultureMech_000307 --> ing1_Air_dried_sieved_calcareous_soil
    ing2_Deionized_water["Deionized water"]
    CultureMech_000307 --> ing2_Deionized_water

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Ammonium magnesium phosphate
H4N.Mg.O4P 137.314 g/mol
0.01 g per vessel VARIABLE CHEBI:149425
ammonium magnesium phosphate
 Place in the base of each vessel before adding soil and water.
2 Air-dried sieved calcareous soil variable VARIABLE Add a layer approximately 1 cm deep to each vessel.
3 Deionized water variable VARIABLE Add carefully to a depth of 7-10 cm above the soil layer.

Preparation

  1. Place approximately 0.01 g ammonium magnesium phosphate in the base of each vessel.
  2. Place a 1 cm layer of air-dried, sieved calcareous soil in each vessel.
  3. Optionally add one grain for organisms that benefit from it.
  4. Add deionized water carefully to a depth of 7-10 cm, then plug or cover.
  5. Autoclave at 15 psi for 15 minutes on each of two consecutive days.
  6. Let stand for at least one further day before inoculation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.807059Z mediadive-import Imported from MediaDive Source: CCAP, ID: C100
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Soil
2026-03-13T06:18:54.340159Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000307
2026-03-17T19:45:05.305061+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "S/W + AMP" to "s_w_amp" for consistent matching
2026-05-16T09:04:31.105341+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-05-20T06:02:04.595326+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7-8' -> {'min': 7.0, 'max': 8.0}
2026-08-25T00:00:00-07:00 repair_ccap_duplicate_compositions.py SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE Rebuilt sw_amp from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM.
2026-09-10T00:00:00-07:00 repair_ccap_sw_flags_score20.py MARKED_CCAP_SW_SPARSE_SCORE20_GRAPH Marked reviewed CCAP S/W soil-water components as intentionally curated with unmapped bulk material identities.