asw_bg

Overview

External ontology

mediadive.medium:C10 (source link unavailable) — ASW:BG

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_ASW_BG.pdf

Composition

flowchart LR
    CultureMech_000316["asw_bg"]
    ing0_Tricine["Tricine"]
    CultureMech_000316 --> ing0_Tricine
    CHEBI_46760["CHEBI:46760"]
    ing0_Tricine -.-> CHEBI_46760
    ing1_NaNO3["NaNO3"]
    CultureMech_000316 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_000316 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000316 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Soil["Soil"]
    CultureMech_000316 --> ing4_Soil
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000316 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000316 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Citric_acid["Citric acid"]
    CultureMech_000316 --> ing7_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing7_Citric_acid -.-> CHEBI_30769
    ing8_Ammonium_ferric_citrate["Ammonium ferric citrate"]
    CultureMech_000316 --> ing8_Ammonium_ferric_citrate
    CHEBI_31604["CHEBI:31604"]
    ing8_Ammonium_ferric_citrate -.-> CHEBI_31604
    ing9_Na2_EDTA["Na2-EDTA"]
    CultureMech_000316 --> ing9_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na2_EDTA -.-> CHEBI_64734
    ing10_Na2CO3["Na2CO3"]
    CultureMech_000316 --> ing10_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing10_Na2CO3 -.-> CHEBI_29377
    ing11_Agar["Agar"]
    CultureMech_000316 --> ing11_Agar
    CHEBI_2509["CHEBI:2509"]
    ing11_Agar -.-> CHEBI_2509
    ing12_H3BO3["H3BO3"]
    CultureMech_000316 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000316 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000316 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_000316 --> ing15_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000316 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing17_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000316 --> ing17_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing17_Co_NO3_2_x_6_H2O -.-> CHEBI_86214

Ingredients (18)

Preparation

1. 1:1 mixture
2. See separate recipes ASW and BG11. This medium is made up in 2 parts which are autoclaved separately and mixed aseptically when cool. Note: vitamins are not required in the ASW part of this recipe.
3. Make up to 1 litre with filtered natural sea water. Adjust the pH to 7.6 – 7.8 with 1N NaOH or 1N HCl. Autoclave at 15 psi.
4. Alternatively use "Ultramarine Synthetica" sea salts 33.6 g and make up to 1 litre with distilled water. Adjust pH as above.
5. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
6. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
7. Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.
8. Make up to 1 litre with deionized water. Adjust pH to 7.1 with 1M NaOH or HCl. For agar add 15.0 g per litre of Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance