3n_bbm_v_recipe_for_customer_orders

CultureMech:000322 bacterial DEFINED SOLID_AGAR
Original name: 3N-BBM+V recipe for customer orders

Overview

External ontology
mediadive.medium:C15 (source link unavailable) — 3N-BBM+V recipe for customer orders
Applications
  • Microbial cultivation
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_3N_BBM_V_customerorders.pdf

Composition

flowchart LR
    CultureMech_000322["3n_bbm_v_recipe_for_customer_orders"]
    ing0_NaNO3["NaNO3"]
    CultureMech_000322 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000322 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000322 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000322 --> ing3_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing4_KH2PO4["KH2PO4"]
    CultureMech_000322 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NaCl["NaCl"]
    CultureMech_000322 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_000322 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Thiamine_hydrochloride["Thiamine hydrochloride"]
    CultureMech_000322 --> ing7_Thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing7_Thiamine_hydrochloride -.-> CHEBI_49105
    ing8_Na2_EDTA["Na2-EDTA"]
    CultureMech_000322 --> ing8_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing8_Na2_EDTA -.-> CHEBI_64734
    ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000322 --> ing9_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000322 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_ZnCl2["ZnCl2"]
    CultureMech_000322 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000322 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_000322 --> ing13_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000322 --> ing14_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing14_Cyanocobalamine -.-> CHEBI_17439

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 NaNO3
NO3.Na 84.994 g/mol
10 G_PER_L CHEBI:63005
sodium nitrate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 KH2PO4
H2O4P.K 136.084 g/mol
2.34 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
7 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
8 Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
1.2 G_PER_L CHEBI:49105
thiamine hydrochloride
9 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.75 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
10 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.097 G_PER_L CHEBI:86254
iron trichloride hexahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.041 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.005 G_PER_L CHEBI:49976
zinc dichloride
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.002 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.004 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
1 G_PER_L CHEBI:17439
cyanocob(III)alamin

Preparation

  1. Once elements are dissolved autoclave at 15 psi for 15 minutes.
  2. Make up to 1 litre with distilled water. For agar add 15 g per litre Bacterialogical Agar. Autoclave at 15 psi for 15 minutes.
  3. Filter sterile
  4. Ensure elements are added in the following sequence:
  5. Once elements are dissolved autoclave at 15 psi for 15 minutes.
  6. Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.517103Z mediadive-import Imported from MediaDive Source: CCAP, ID: C15
2026-03-13T06:18:54.444108Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000322
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.421608+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "3N-BBM+V recipe for customer orders" to "3n_bbm_v_recipe_for_customer_orders" for consistent matching
2026-06-05T07:40:15.821212+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.555350+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.