bg11

CultureMech:000325 bacterial DEFINED LIQUID
Original name: BG11₀

Overview

External ontology
mediadive.medium:C18 (source link unavailable) — BG11₀
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_BG110.pdf

Composition

flowchart LR
    CultureMech_000325["bg11"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_000325 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000325 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000325 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Citric_acid["Citric acid"]
    CultureMech_000325 --> ing3_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing3_Citric_acid -.-> CHEBI_30769
    ing4_Ammonium_ferric_citrate["Ammonium ferric citrate"]
    CultureMech_000325 --> ing4_Ammonium_ferric_citrate
    CHEBI_31604["CHEBI:31604"]
    ing4_Ammonium_ferric_citrate -.-> CHEBI_31604
    ing5_Na2_EDTA["Na2-EDTA"]
    CultureMech_000325 --> ing5_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing5_Na2_EDTA -.-> CHEBI_64734
    ing6_Na2CO3["Na2CO3"]
    CultureMech_000325 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_H3BO3["H3BO3"]
    CultureMech_000325 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000325 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000325 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000325 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000325 --> ing11_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing12_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000325 --> ing12_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing12_Co_NO3_2_x_6_H2O -.-> CHEBI_86214

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
10 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
10 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
10 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Citric acid
C6H8O7 192.123 g/mol
10 G_PER_L CHEBI:30769
citric acid
5 Ammonium ferric citrate 10 G_PER_L CHEBI:31604
ferric ammonium citrate
6 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
10 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
7 Na2CO3
CO3.2Na 105.988 g/mol
25.3 G_PER_L CHEBI:29377
sodium carbonate
8 H3BO3
H3BO3 61.833 g/mol
2.86 G_PER_L CHEBI:33118
boric acid
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.81 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.22 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.39 G_PER_L CHEBI:75213
sodium molybdate dihydrate
12 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.08 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
13 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.05 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate

Preparation

  1. This medium is standard BG11 but omitting NaNO3.
  2. Make up to 1 litre with deionized water. Adjust pH to 7.1 with 1M NaOH or HCl. For agar add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.
  3. Due to precipitation, larger volumes require stocks of citric acid and ammonium ferric citrate to be autoclaved separately in 100ml deionized water or alternatively they can be autoclaved separately in test tubes and added to sterile medium in the airflow cabinet.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.530283Z mediadive-import Imported from MediaDive Source: CCAP, ID: C18
2026-03-13T06:18:54.473656Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000325
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.454307+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BG11₀" to "bg11" for consistent matching
2026-06-05T07:40:16.004792+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.597888+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.