c_medium_modified

CultureMech:000328 bacterial DEFINED SOLID_AGAR
Original name: C Medium, Modified

Overview

External ontology
mediadive.medium:C20 (source link unavailable) — C Medium, Modified
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_C_Modified.pdf

Composition

flowchart LR
    CultureMech_000328["c_medium_modified"]
    ing0_KNO3["KNO3"]
    CultureMech_000328 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000328 --> ing1_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing1_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing2_Sodium_glycerophosphate["Sodium glycerophosphate"]
    CultureMech_000328 --> ing2_Sodium_glycerophosphate
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000328 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Tris_hydroxymethyl_aminomethane["Tris(hydroxymethyl)aminomethane"]
    CultureMech_000328 --> ing4_Tris_hydroxymethyl_aminomethane
    CHEBI_9754["CHEBI:9754"]
    ing4_Tris_hydroxymethyl_aminomethane -.-> CHEBI_9754
    ing5_Agar["Agar"]
    CultureMech_000328 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Na2_EDTA["Na2-EDTA"]
    CultureMech_000328 --> ing6_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing6_Na2_EDTA -.-> CHEBI_64734
    ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000328 --> ing7_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000328 --> ing8_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing9_ZnCl2_x_6_H2O["ZnCl2 x 6 H2O"]
    CultureMech_000328 --> ing9_ZnCl2_x_6_H2O
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000328 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000328 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_Thiamine_hydrochloride["Thiamine hydrochloride"]
    CultureMech_000328 --> ing12_Thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing12_Thiamine_hydrochloride -.-> CHEBI_49105
    ing13_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000328 --> ing13_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing13_Cyanocobalamine -.-> CHEBI_17439
    ing14_Biotin["Biotin"]
    CultureMech_000328 --> ing14_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing14_Biotin -.-> CHEBI_15956

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
0.1 G_PER_L CHEBI:63043
potassium nitrate
2 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
0.15 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
3 Sodium glycerophosphate 0.05 G_PER_L
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.04 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Tris(hydroxymethyl)aminomethane
C4H11NO3 121.136 g/mol
0.5 G_PER_L CHEBI:9754
tris
6 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
7 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.75 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
8 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.097 G_PER_L CHEBI:86254
iron trichloride hexahydrate
9 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.041 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
10 ZnCl2 x 6 H2O 0.005 G_PER_L
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.002 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.004 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
1.2 G_PER_L CHEBI:49105
thiamine hydrochloride
14 Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
1 G_PER_L CHEBI:17439
cyanocob(III)alamin
15 Biotin
C10H16N2O3S 244.316 g/mol
0.05 G_PER_L CHEBI:15956
biotin

Preparation

  1. Dissolve the TRIS buffer into 900 ml distilled water, then add the remaining components. Bring to 1 litre with distilled water. For agar add 15 g per litre Bacterial Agar. Autoclave at 15 psi for 15 minutes. Final pH should be 7.5.
  2. Add to 1000 ml of distilled water 0.75 g Na2EDTA and the minerals in exactly the following sequence:
  3. Filter sterile
  4. Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.
  5. Filter sterile.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.537532Z mediadive-import Imported from MediaDive Source: CCAP, ID: C20
2026-03-13T06:18:54.501006Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000328
2026-03-17T06:30:35.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.485425+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "C Medium, Modified" to "c_medium_modified" for consistent matching
2026-06-05T07:40:51.946597+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.604148+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:54.065405+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.