Overview
- External ontology
- mediadive.medium:C21 (source link unavailable) — CGM
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_CGM.pdf
Composition
flowchart LR
CultureMech_000329["cgm"]
ing0_Proteose_peptone["Proteose peptone"]
CultureMech_000329 --> ing0_Proteose_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_000329 --> ing1_Yeast_extract
ing2_D_Glucose["D-Glucose"]
CultureMech_000329 --> ing2_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing2_D_Glucose -.-> CHEBI_17634
ing3_NaNO3["NaNO3"]
CultureMech_000329 --> ing3_NaNO3
CHEBI_63005["CHEBI:63005"]
ing3_NaNO3 -.-> CHEBI_63005
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000329 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000329 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_000329 --> ing6_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing7_KH2PO4["KH2PO4"]
CultureMech_000329 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_NaCl["NaCl"]
CultureMech_000329 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_Agar["Agar"]
CultureMech_000329 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_Na2_EDTA["Na2-EDTA"]
CultureMech_000329 --> ing10_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing10_Na2_EDTA -.-> CHEBI_64734
ing11_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000329 --> ing11_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing11_FeCl3_x_6_H2O -.-> CHEBI_86254
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000329 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_ZnCl2["ZnCl2"]
CultureMech_000329 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000329 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
CultureMech_000329 --> ing15_Na2_MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
ing16_Thiamine_hydrochloride["Thiamine hydrochloride"]
CultureMech_000329 --> ing16_Thiamine_hydrochloride
CHEBI_49105["CHEBI:49105"]
ing16_Thiamine_hydrochloride -.-> CHEBI_49105
ing17_Cyanocobalamine["Cyanocobalamine"]
CultureMech_000329 --> ing17_Cyanocobalamine
CHEBI_17439["CHEBI:17439"]
ing17_Cyanocobalamine -.-> CHEBI_17439
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Proteose peptone | 0.25 G_PER_L | ||
| 2 | Yeast extract | 0.25 G_PER_L | ||
| 3 |
D-Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 4 |
NaNO3
NO3.Na 84.994 g/mol
|
10 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
10 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
10 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
23.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 11 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.75 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 12 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.097 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.041 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.005 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.002 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
Na2 MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.004 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
Thiamine hydrochloride
C12H17N4OS.Cl.HCl 337.276 g/mol
|
1.2 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 18 |
Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
|
1 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Preparation
- Once elements are dissolved autoclave at 15 psi for 15 minutes.
- Make up to 1 litre with distilled water. For agar add 15 g per litre Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.
- Ensure elements are added in the following sequence:
- Once elements are dissolved autoclave at 15 psi for 15 minutes.
- Filter sterile
- Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.541128Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C21 |
| 2026-03-13T06:18:54.509843Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000329 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.495558+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CGM" to "cgm" for consistent matching |
| 2026-06-05T07:40:53.020655+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.441994+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |