Overview
- External ontology
- mediadive.medium:C22 (source link unavailable) — CH
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_CH.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_CH.pdf on 2026-08-25.
Composition
flowchart LR
CultureMech_000330["ch"]
ing0_Deionized_water["Deionized water"]
CultureMech_000330 --> ing0_Deionized_water
CHEBI_15377["CHEBI:15377"]
ing0_Deionized_water -.-> CHEBI_15377
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Deionized water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Add 5 ml of each stock solution and make up to 1 L with deionized water.
- Autoclave at 15 psi for 15 minutes.
Solutions
NaCl stock solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 20 G_PER_L |
Prepare with 2.0 g NaCl per 100 ml; add 5 ml/L.
KCl stock solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KCl | 0.8 G_PER_L |
Prepare with 0.08 g KCl per 100 ml; add 5 ml/L.
CaCl2 stock solution
Dose: 5.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 | 1.2 G_PER_L |
Prepare with 0.12 g CaCl2 per 100 ml; add 5 ml/L.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.544907Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C22 |
| 2026-03-13T06:18:54.514758Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000330 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.501310+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CH" to "ch" for consistent matching |
| 2026-06-05T07:40:53.034960+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-25T00:00:00-07:00 | repair_ccap_duplicate_compositions.py | SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE | Rebuilt ch from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM. |