dm

CultureMech:000333 bacterial DEFINED LIQUID
Original name: DM

Overview

External ontology
mediadive.medium:C25 (source link unavailable) — DM
Applications
  • Microbial cultivation
pH
6.9
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_DM.pdf

Composition

flowchart LR
    CultureMech_000333["dm"]
    ing0_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000333 --> ing0_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing0_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000333 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000333 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaHCO3["NaHCO3"]
    CultureMech_000333 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_000333 --> ing4_FeNa_EDTA
    ing5_Na2_EDTA["Na2-EDTA"]
    CultureMech_000333 --> ing5_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing5_Na2_EDTA -.-> CHEBI_64734
    ing6_H3BO3["H3BO3"]
    CultureMech_000333 --> ing6_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing6_H3BO3 -.-> CHEBI_33118
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000333 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000333 --> ing8__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing8__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing9_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000333 --> ing9_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing9_Cyanocobalamine -.-> CHEBI_17439
    ing10_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000333 --> ing10_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing10_Thiamine_HCl -.-> CHEBI_49105
    ing11_Biotin["Biotin"]
    CultureMech_000333 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
    CultureMech_000333 --> ing12_Na2SiO3_x_9_H2O
    CHEBI_132108["CHEBI:132108"]
    ing12_Na2SiO3_x_9_H2O -.-> CHEBI_132108

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
1 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
2.34 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
5 FeNa-EDTA 2.25 G_PER_L
6 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
2.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
7 H3BO3
H3BO3 61.833 g/mol
2.48 G_PER_L CHEBI:33118
boric acid
8 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.39 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
9 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
1 G_PER_L CHEBI:91249
ammonium molybdate
10 Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
0.04 G_PER_L CHEBI:17439
cyanocob(III)alamin
11 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.04 G_PER_L CHEBI:49105
thiamine hydrochloride
12 Biotin
C10H16N2O3S 244.316 g/mol
0.04 G_PER_L CHEBI:15956
biotin
13 Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
57 G_PER_L CHEBI:132108
sodium silicate nonahydrate

Preparation

  1. Make up to 1 litre with deionized water. Adjust to pH 6.9 with 1M NaOH or 1M HCl, prior to autoclaving. Autoclave at 15 psi for 15 minutes.
  2. Silicate can react with glass, ensure this stock solution is stored in plastic bottles.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.549760Z mediadive-import Imported from MediaDive Source: CCAP, ID: C25
2026-03-13T06:18:54.524731Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000333
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.512987+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DM" to "dm" for consistent matching
2026-06-05T07:40:58.772257+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.422865+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.