Overview
- External ontology
- mediadive.medium:C26 (source link unavailable) — EG
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_EG.pdf
Composition
flowchart LR
CultureMech_000334["eg"]
ing0_Sodium_acetate_x_3_H2O["Sodium acetate x 3 H2O"]
CultureMech_000334 --> ing0_Sodium_acetate_x_3_H2O
CHEBI_32954["CHEBI:32954"]
ing0_Sodium_acetate_x_3_H2O -.-> CHEBI_32954
ing1_Lab_Lemco_powder["Lab Lemco powder"]
CultureMech_000334 --> ing1_Lab_Lemco_powder
ing2_Tryptone["Tryptone"]
CultureMech_000334 --> ing2_Tryptone
ing3_Yeast_extract["Yeast extract"]
CultureMech_000334 --> ing3_Yeast_extract
ing4_CaCl2["CaCl2"]
CultureMech_000334 --> ing4_CaCl2
CHEBI_3312["CHEBI:3312"]
ing4_CaCl2 -.-> CHEBI_3312
ing5_Agar["Agar"]
CultureMech_000334 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium acetate x 3 H2O
C2H3O2.Na 82.034 g/mol
|
1 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 2 | Lab Lemco powder | 1 G_PER_L | ||
| 3 | Tryptone | 2 G_PER_L | ||
| 4 | Yeast extract | 2 G_PER_L | ||
| 5 |
CaCl2
Ca.2Cl 110.984 g/mol
|
10 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Add constituents above and make up to 1 litre with deionized water. For agar add 15 g per litre Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.552375Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C26 |
| 2026-03-13T06:18:54.529219Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000334 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.518342+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "EG" to "eg" for consistent matching |
| 2026-06-05T07:40:59.395186+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |