eg_jm

CultureMech:000335 bacterial COMPLEX SOLID_AGAR
Original name: EG:JM

Overview

External ontology
mediadive.medium:C27 (source link unavailable) — EG:JM
Applications
  • Microbial cultivation
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_EG_JM.pdf

Composition

flowchart LR
    CultureMech_000335["eg_jm"]
    ing0_Sodium_acetate_x_3_H2O["Sodium acetate x 3 H2O"]
    CultureMech_000335 --> ing0_Sodium_acetate_x_3_H2O
    CHEBI_32954["CHEBI:32954"]
    ing0_Sodium_acetate_x_3_H2O -.-> CHEBI_32954
    ing1_Lab_Lemco_powder["Lab Lemco powder"]
    CultureMech_000335 --> ing1_Lab_Lemco_powder
    ing2_Tryptone["Tryptone"]
    CultureMech_000335 --> ing2_Tryptone
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_000335 --> ing3_Yeast_extract
    ing4_CaCl2["CaCl2"]
    CultureMech_000335 --> ing4_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing4_CaCl2 -.-> CHEBI_3312
    ing5_Agar["Agar"]
    CultureMech_000335 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000335 --> ing6_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing6_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing7_KH2PO4["KH2PO4"]
    CultureMech_000335 --> ing7_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing7_KH2PO4 -.-> CHEBI_63036
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000335 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_NaHCO3["NaHCO3"]
    CultureMech_000335 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_NaNO3["NaNO3"]
    CultureMech_000335 --> ing10_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing10_NaNO3 -.-> CHEBI_63005
    ing11_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_000335 --> ing11_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing11_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing12_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_000335 --> ing12_FeNa_EDTA
    ing13_Na2_EDTA["Na2-EDTA"]
    CultureMech_000335 --> ing13_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing13_Na2_EDTA -.-> CHEBI_64734
    ing14_H3BO3["H3BO3"]
    CultureMech_000335 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000335 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000335 --> ing16__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing16__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing17_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000335 --> ing17_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing17_Cyanocobalamine -.-> CHEBI_17439
    ing18_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000335 --> ing18_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing18_Thiamine_HCl -.-> CHEBI_49105
    ing19_Biotine["Biotine"]
    CultureMech_000335 --> ing19_Biotine
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotine -.-> CHEBI_15956

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 Sodium acetate x 3 H2O
C2H3O2.Na 82.034 g/mol
1 G_PER_L CHEBI:32954
sodium acetate
2 Lab Lemco powder 1 G_PER_L
3 Tryptone 2 G_PER_L
4 Yeast extract 2 G_PER_L
5 CaCl2
Ca.2Cl 110.984 g/mol
10 G_PER_L CHEBI:3312
calcium dichloride
6 Agar 15.0 G_PER_L CHEBI:2509
agar
 (for solid medium) [Collapsed 2 identical duplicates: 15.0, 15.0]
7 Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
1 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
8 KH2PO4
H2O4P.K 136.084 g/mol
2.34 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
10 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
12 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
1 G_PER_L CHEBI:34683
disodium hydrogenphosphate
13 FeNa-EDTA 2.25 G_PER_L
14 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
2.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
15 H3BO3
H3BO3 61.833 g/mol
2.48 G_PER_L CHEBI:33118
boric acid
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.39 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 (NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
1 G_PER_L CHEBI:91249
ammonium molybdate
18 Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
0.04 G_PER_L CHEBI:17439
cyanocob(III)alamin
19 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.04 G_PER_L CHEBI:49105
thiamine hydrochloride
20 Biotine
C10H16N2O3S 244.316 g/mol
0.04 G_PER_L CHEBI:15956
biotin

Preparation

  1. 1:1 mixture. See separate recipes. Mix then autoclave at 15 psi for 15 minutes.
  2. Add constituents above and make up to 1 litre with deionized water. For agar add 15 g per litre Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.
  3. Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacterial Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.554186Z mediadive-import Imported from MediaDive Source: CCAP, ID: C27
2026-03-13T03:09:02.595165Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:54.536288Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000335
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.526040+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "EG:JM" to "eg_jm" for consistent matching
2026-06-05T07:40:59.403953+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.652243+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:38:04.269021+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.