e26_biotin

Overview

External ontology

mediadive.medium:C28 (source link unavailable) — E26 + Biotin

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_E26_Biotin.pdf

Composition

flowchart LR
    CultureMech_000336["e26_biotin"]
    ing0_Natural_sea_water["Natural sea water"]
    CultureMech_000336 --> ing0_Natural_sea_water
    ing1_KNO3["KNO3"]
    CultureMech_000336 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000336 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000336 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000336 --> ing4_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing4_Cyanocobalamine -.-> CHEBI_17439
    ing5_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000336 --> ing5_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing5_Thiamine_HCl -.-> CHEBI_49105
    ing6_Biotin["Biotin"]
    CultureMech_000336 --> ing6_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing6_Biotin -.-> CHEBI_15956
    ing7_Soil["Soil"]
    CultureMech_000336 --> ing7_Soil

Ingredients (8)

Preparation

1. This medium is made up in 2 parts which are autoclaved separately and mixed aseptically, (1:1), when cool. This is to avoid precipitation.
2. Alternatively, use 17.5 g of "Ultramarine Synthetica" sea salts in 500 ml of distilled water.
3. Distilled water to 500 ml. Autoclave
4. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
5. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
6. Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Target organisms

No target organisms are recorded.

Provenance