e26_biotin_ant

Overview

External ontology

mediadive.medium:C29 (source link unavailable) — E26 + Biotin:ANT

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_E26_Biotin_ANT.pdf

Composition

flowchart LR
    CultureMech_000337["e26_biotin_ant"]
    ing0_Natural_sea_water["Natural sea water"]
    CultureMech_000337 --> ing0_Natural_sea_water
    ing1_KNO3["KNO3"]
    CultureMech_000337 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000337 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000337 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000337 --> ing4_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing4_Cyanocobalamine -.-> CHEBI_17439
    ing5_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000337 --> ing5_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing5_Thiamine_HCl -.-> CHEBI_49105
    ing6_Biotin["Biotin"]
    CultureMech_000337 --> ing6_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing6_Biotin -.-> CHEBI_15956
    ing7_Soil["Soil"]
    CultureMech_000337 --> ing7_Soil
    ing8_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_000337 --> ing8_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing8_NaH2PO4_x_2_H2O -.-> CHEBI_37585
    ing9_Tris["Tris"]
    CultureMech_000337 --> ing9_Tris
    CHEBI_9754["CHEBI:9754"]
    ing9_Tris -.-> CHEBI_9754
    ing10_Glycine["Glycine"]
    CultureMech_000337 --> ing10_Glycine
    CHEBI_15428["CHEBI:15428"]
    ing10_Glycine -.-> CHEBI_15428
    ing11_Biotine["Biotine"]
    CultureMech_000337 --> ing11_Biotine
    ing11_Biotine -.-> CHEBI_15956
    ing12_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_000337 --> ing12_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing12_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing13_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000337 --> ing13_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing13_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing14_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_000337 --> ing14_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing14_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000337 --> ing15_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000337 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000337 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000337 --> ing18_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing18_CoSO4_x_7_H2O -.-> CHEBI_91244

Ingredients (19)

Preparation

1. E26 + biotin medium and Antia's medium are made up separately then mixed 1:1.
2. This medium is made up in 2 parts which are autoclaved separately and mixed aseptically, (1:1), when cool. This is to avoid precipitation.
3. Alternatively, use 17.5 g of "Ultramarine Synthetica" sea salts in 500 ml of distilled water.
4. Distilled water to 500 ml. Autoclave
5. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
6. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
7. Soil is prepared as above. Air­-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and filtered through Whatman No 1 filter paper, then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.
8. Make up to 1 litre with distilled water and autoclave at 15 psi. Final pH should be 7.6 -­ 7.8.
9. Make up to 1 litre with distilled water and adjust pH to 7.6 ­- 7.8 with dilute HCl or NaOH. Store frozen.

Target organisms

No target organisms are recorded.

Provenance