ajs

Overview

External ontology

mediadive.medium:C2 (source link unavailable) — AJS

Applications

• Microbial cultivation

pH

1.5

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_AJS.pdf

Composition

flowchart LR
    CultureMech_000338["ajs"]
    ing0_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000338 --> ing0_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing0_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000338 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000338 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NaHCO3["NaHCO3"]
    CultureMech_000338 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_NaNO3["NaNO3"]
    CultureMech_000338 --> ing4_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing4_NaNO3 -.-> CHEBI_63005
    ing5_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_000338 --> ing5_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing5_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing6_Agar["Agar"]
    CultureMech_000338 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Soil["Soil"]
    CultureMech_000338 --> ing7_Soil
    ing8_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_000338 --> ing8_FeNa_EDTA
    ing9_Na2_EDTA["Na2-EDTA"]
    CultureMech_000338 --> ing9_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na2_EDTA -.-> CHEBI_64734
    ing10_H3BO3["H3BO3"]
    CultureMech_000338 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000338 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
    CultureMech_000338 --> ing12__NH4_6Mo7O24_x_4_H2O
    CHEBI_91249["CHEBI:91249"]
    ing12__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
    ing13_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000338 --> ing13_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing13_Cyanocobalamine -.-> CHEBI_17439
    ing14_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000338 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Biotine["Biotine"]
    CultureMech_000338 --> ing15_Biotine
    CHEBI_15956["CHEBI:15956"]
    ing15_Biotine -.-> CHEBI_15956

Ingredients (16)

Preparation

1. Acidified 97:3 mixture of JM and SE2
2. See separate recipes. For approximately 1 litre of final medium, mix 970 ml JM with 30 ml SE2. Add 10 ml of concentrated H2SO4 to give a pH of approximately 1.5. Autoclave at 15 psi for 15 minutes.
3. Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacterial Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.
4. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
5. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and hand picked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry it may be sieved through a finer mesh (2­4 mm) or stored as it is prior to use.
6. Soil is prepared as above. 105 g of air­dried sieved soil and 660 ml of deionized water are placed in a 1 litre bottle and autoclaved once at 15 psi for 15 minutes, then again after 24 hours. The contents of the bottle are left to settle (usually for at least a week) and then the supernatant is decanted and filtered. The final pH should be 7.0 - 8.0.

Target organisms

No target organisms are recorded.

Provenance