f_10

CultureMech:000345 bacterial COMPLEX SOLID_AGAR
Original name: f/10

Overview

External ontology
mediadive.medium:C36 (source link unavailable) — f/10
Applications
  • Microbial cultivation
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_f2.pdf

Composition

flowchart LR
    CultureMech_000345["f_10"]
    ing0_Natural_sea_water["Natural sea water"]
    CultureMech_000345 --> ing0_Natural_sea_water
    ing1_NaNO3["NaNO3"]
    CultureMech_000345 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_000345 --> ing2_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing2_NaH2PO4_x_2_H2O -.-> CHEBI_37585
    ing3_Agar["Agar"]
    CultureMech_000345 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Na2_EDTA["Na2-EDTA"]
    CultureMech_000345 --> ing4_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing4_Na2_EDTA -.-> CHEBI_64734
    ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000345 --> ing5_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing6_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000345 --> ing6_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing6_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000345 --> ing7_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000345 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000345 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000345 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000345 --> ing11_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing11_Cyanocobalamine -.-> CHEBI_17439
    ing12_Biotin["Biotin"]
    CultureMech_000345 --> ing12_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing12_Biotin -.-> CHEBI_15956
    ing13_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000345 --> ing13_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing13_Thiamine_HCl -.-> CHEBI_49105

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Natural sea water 1800.0 G_PER_L [Merged 2 duplicates: 800.0, 1000.0]
2 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
3 NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
1 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
4 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
5 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
41.6 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
6 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
31.5 G_PER_L CHEBI:86254
iron trichloride hexahydrate
7 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
8 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.22 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
9 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.1 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.8 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.06 G_PER_L CHEBI:75213
sodium molybdate dihydrate
12 Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
1 G_PER_L CHEBI:17439
cyanocob(III)alamin
13 Biotin
C10H16N2O3S 244.316 g/mol
1 G_PER_L CHEBI:15956
biotin
14 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.1 G_PER_L CHEBI:49105
thiamine hydrochloride

Preparation

  1. For f/10 medium for calcifying Emiliania, simply dilute all stock solutions by 1/5th.
  2. Make up to 1 litre with filtered natural seawater. Adjust pH to 8.0 with 1M NaOH or 1 M HCl prior to autoclaving. For agar add 15g per litre Bacteriological Agar. Autoclave at 15 psi for 15 minutes.
  3. First make primary stocks of Cyanocobalamin and Biotin.
  4. Dispense any excess primary stocks into 1 ml aliquots and freeze.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.591377Z mediadive-import Imported from MediaDive Source: CCAP, ID: C36
2026-03-13T03:08:45.915710Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:54.624177Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000345
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.616397+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "f/10" to "f_10" for consistent matching
2026-06-05T07:40:59.793905+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.