Overview
- External ontology
- mediadive.medium:C40 (source link unavailable) — JM
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_JM.pdf
Composition
flowchart LR
CultureMech_000350["jm"]
ing0_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_000350 --> ing0_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing0_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing1_KH2PO4["KH2PO4"]
CultureMech_000350 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000350 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NaHCO3["NaHCO3"]
CultureMech_000350 --> ing3_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing3_NaHCO3 -.-> CHEBI_32139
ing4_NaNO3["NaNO3"]
CultureMech_000350 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_000350 --> ing5_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing6_Agar["Agar"]
CultureMech_000350 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_FeNa_EDTA["FeNa-EDTA"]
CultureMech_000350 --> ing7_FeNa_EDTA
ing8_Na2_EDTA["Na2-EDTA"]
CultureMech_000350 --> ing8_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing8_Na2_EDTA -.-> CHEBI_64734
ing9_H3BO3["H3BO3"]
CultureMech_000350 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000350 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_000350 --> ing11__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing11__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing12_Cyanocobalamine["Cyanocobalamine"]
CultureMech_000350 --> ing12_Cyanocobalamine
CHEBI_17439["CHEBI:17439"]
ing12_Cyanocobalamine -.-> CHEBI_17439
ing13_Thiamine_HCl["Thiamine HCl"]
CultureMech_000350 --> ing13_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_HCl -.-> CHEBI_49105
ing14_Biotine["Biotine"]
CultureMech_000350 --> ing14_Biotine
CHEBI_15956["CHEBI:15956"]
ing14_Biotine -.-> CHEBI_15956
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
1 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.34 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 6 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
1 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 8 | FeNa-EDTA | 2.25 G_PER_L | ||
| 9 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
2.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
2.48 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.39 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
1 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 13 |
Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
|
0.04 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
|
| 14 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.04 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 15 |
Biotine
C10H16N2O3S 244.316 g/mol
|
0.04 G_PER_L |
CHEBI:15956
biotin
|
Preparation
- Make up to 1 litre with deionized water. For agar, add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.606448Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C40 |
| 2026-03-13T06:18:54.660997Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000350 |
| 2026-03-17T06:29:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.656435+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JM" to "jm" for consistent matching |
| 2026-06-05T07:41:10.215788+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.320727+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |