l1

CultureMech:000355 bacterial COMPLEX SOLID_AGAR
Original name: L1

Overview

External ontology
mediadive.medium:C45 (source link unavailable) — L1
Applications
  • Microbial cultivation
pH
8.0
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_L1.pdf

Composition

flowchart LR
    CultureMech_000355["l1"]
    ing0_NaNO3["NaNO3"]
    CultureMech_000355 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
    CultureMech_000355 --> ing1_NaH2PO4_x_2_H2O
    CHEBI_37585["CHEBI:37585"]
    ing1_NaH2PO4_x_2_H2O -.-> CHEBI_37585
    ing2_Natural_sea_water["Natural sea water"]
    CultureMech_000355 --> ing2_Natural_sea_water
    ing3_Agar["Agar"]
    CultureMech_000355 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_000355 --> ing4_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing4_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000355 --> ing5_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing6_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000355 --> ing6_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing6_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000355 --> ing7_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000355 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000355 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000355 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H2SeO3["H2SeO3"]
    CultureMech_000355 --> ing11_H2SeO3
    CHEBI_26642["CHEBI:26642"]
    ing11_H2SeO3 -.-> CHEBI_26642
    ing12_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
    CultureMech_000355 --> ing12_NiSO4_x_6_H2O
    CHEBI_53001["CHEBI:53001"]
    ing12_NiSO4_x_6_H2O -.-> CHEBI_53001
    ing13_Na3VO4["Na3VO4"]
    CultureMech_000355 --> ing13_Na3VO4
    CHEBI_35607["CHEBI:35607"]
    ing13_Na3VO4 -.-> CHEBI_35607
    ing14_K2CrO4["K2CrO4"]
    CultureMech_000355 --> ing14_K2CrO4
    CHEBI_75249["CHEBI:75249"]
    ing14_K2CrO4 -.-> CHEBI_75249
    ing15_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000355 --> ing15_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing15_Thiamine_HCl -.-> CHEBI_49105
    ing16_Cyanocobalamin["Cyanocobalamin"]
    CultureMech_000355 --> ing16_Cyanocobalamin
    CHEBI_17439["CHEBI:17439"]
    ing16_Cyanocobalamin -.-> CHEBI_17439
    ing17_Biotin["Biotin"]
    CultureMech_000355 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 NaNO3
NO3.Na 84.994 g/mol
1 G_PER_L CHEBI:63005
sodium nitrate
2 NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
1 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
3 Natural sea water 1000 G_PER_L
4 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
5 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
4.36 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
6 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
3.14 G_PER_L CHEBI:86254
iron trichloride hexahydrate
7 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.125 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
8 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
1.5 G_PER_L CHEBI:75213
sodium molybdate dihydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.5 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.5 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.5 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H2SeO3
H2O3Se 128.973 g/mol
3.004 G_PER_L CHEBI:26642
selenous acid
13 NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
0.5 G_PER_L CHEBI:53001
nickel sulfate
14 Na3VO4
3Na.O4V 183.91 g/mol
0.5 G_PER_L CHEBI:35607
trisodium vanadate
15 K2CrO4
CrO4.2K 194.188 g/mol
0.5 G_PER_L CHEBI:75249
potassium chromate
16 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.5 G_PER_L CHEBI:49105
thiamine hydrochloride
17 Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
1 G_PER_L CHEBI:17439
cyanocob(III)alamin
18 Biotin
C10H16N2O3S 244.316 g/mol
1 G_PER_L CHEBI:15956
biotin

Preparation

  1. Make up to 1 litre with filtered natural seawater. Adjust pH to 8.0 with 1M NaOH or 1 M HCl prior to autoclaving. For agar add 15g per litre Bacteriological Agar. Autoclave at 15 psi for 15 minutes.
  2. There are 11 chemicals. Prepare Primary stock solutions first. Add first two chemicals separately in 500ml/dH2O, allowing each to completely dissolve before adding the primary stocks in order:
  3. First make primary stocks of Cyanocobalamin and Biotin.
  4. Dispense any excess primary stocks into 1 ml aliquots and freeze.
  5. For final vitamin mix stock solution:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.626182Z mediadive-import Imported from MediaDive Source: CCAP, ID: C45
2026-01-28T19:48:33.873611Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:18:54.703759Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000355
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.704531+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "L1" to "l1" for consistent matching
2026-06-05T07:41:10.605871+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.