maf6_se

Overview

External ontology

mediadive.medium:C46 (source link unavailable) — MAF6:SE

Applications

• Microbial cultivation

pH

6.6

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_MAF6_SE.pdf

Composition

flowchart LR
    CultureMech_000356["maf6_se"]
    ing0_NaNO3["NaNO3"]
    CultureMech_000356 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_NH4NO3["NH4NO3"]
    CultureMech_000356 --> ing1_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing1_NH4NO3 -.-> CHEBI_63038
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000356 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
    CultureMech_000356 --> ing3_K2HPO4_x_3_H2O
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4_x_3_H2O -.-> CHEBI_131527
    ing4_KH2PO4["KH2PO4"]
    CultureMech_000356 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000356 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MES["MES"]
    CultureMech_000356 --> ing6_MES
    CHEBI_39010["CHEBI:39010"]
    ing6_MES -.-> CHEBI_39010
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_000356 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000356 --> ing8_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing8_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000356 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_ZnCl2_x_6_H2O["ZnCl2 x 6 H2O"]
    CultureMech_000356 --> ing10_ZnCl2_x_6_H2O
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000356 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000356 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_Thiamine_hydrochloride["Thiamine hydrochloride"]
    CultureMech_000356 --> ing13_Thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing13_Thiamine_hydrochloride -.-> CHEBI_49105
    ing14_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000356 --> ing14_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing14_Cyanocobalamine -.-> CHEBI_17439
    ing15_Soil["Soil"]
    CultureMech_000356 --> ing15_Soil

Ingredients (16)

Preparation

1. Make up to 1 litre with distilled water. pH is adjusted to 6.6. Autoclave at 15 psi for 15 minutes.
2. Add to 1000 ml of distilled water 0.75 g Na2EDTA and the minerals in exactly the following sequence:
3. Filter sterile
4. Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.
5. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
6. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and hand picked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry it may be sieved through a finer mesh (2­4 mm) or stored as it is prior to use.
7. Soil is prepared as above. 105 g of air­dried sieved soil and 660 ml of deionized water are placed in a 1 litre bottle and autoclaved once at 15 psi for 15 minutes, then again after 24 hours. The contents of the bottle are left to settle (usually for at least a week) and then the supernatant is decanted and filtered. The final pH should be 7.0 - 8.0.

Target organisms

No target organisms are recorded.

Provenance