Overview
- External ontology
- mediadive.medium:C53 (source link unavailable) — MErds
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_MErds.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_MErds.pdf on 2026-08-25.
Composition
flowchart LR
CultureMech_000364["merds"]
ing0_Filtered_natural_seawater["Filtered natural seawater"]
CultureMech_000364 --> ing0_Filtered_natural_seawater
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Filtered natural seawater | 1 L | Make the final medium up to 1 L after adding the stocks. |
Preparation
- Add SES medium and both salt stocks, then make up to 1 L with filtered natural seawater.
- Autoclave at 15 psi for 15 minutes.
- Filter the final medium if precipitation is problematic.
Solutions
SES medium
Dose: 100.00 ML_PER_L
Use the separately defined CCAP SES recipe.
NaNO3 stock solution
Dose: 2.65 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaNO3 | 75 G_PER_L |
Prepare with 7.5 g NaNO3 per 100 ml; add 2.65 ml/L.
Na2HPO4.2H2O stock solution
Dose: 0.62 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2HPO4.2H2O | 20 G_PER_L |
Prepare with 2.0 g Na2HPO4.2H2O per 100 ml; add 0.62 ml/L.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.654900Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C53 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Soil; Added default concentration for ingredient: Soil |
| 2026-03-13T03:09:15.378114Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:54.771044Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000364 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.777351+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MErds" to "merds" for consistent matching |
| 2026-05-16T09:04:21.200573+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:30.469934+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.453966+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:06.573469+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-25T00:00:00-07:00 | repair_ccap_duplicate_compositions.py | SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE | Rebuilt merds from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM. |