mhy

Overview

External ontology

mediadive.medium:C55 (source link unavailable) — MHY

Applications

• Microbial cultivation

pH

6.8

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_MHY.pdf

Composition

flowchart LR
    CultureMech_000366["mhy"]
    ing0_KNO3["KNO3"]
    CultureMech_000366 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000366 --> ing1_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing1_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000366 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_K2CO3["K2CO3"]
    CultureMech_000366 --> ing3_K2CO3
    CHEBI_131526["CHEBI:131526"]
    ing3_K2CO3 -.-> CHEBI_131526
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000366 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Na2_EDTA["Na2-EDTA"]
    CultureMech_000366 --> ing5_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing5_Na2_EDTA -.-> CHEBI_64734
    ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000366 --> ing6_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000366 --> ing7_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing8_ZnCl2["ZnCl2"]
    CultureMech_000366 --> ing8_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing8_ZnCl2 -.-> CHEBI_49976
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000366 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_Na2_MoO4_x_2_H2O["Na2 MoO4 x 2 H2O"]
    CultureMech_000366 --> ing10_Na2_MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2_MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_Thiamine_hydrochloride["Thiamine hydrochloride"]
    CultureMech_000366 --> ing11_Thiamine_hydrochloride
    CHEBI_49105["CHEBI:49105"]
    ing11_Thiamine_hydrochloride -.-> CHEBI_49105
    ing12_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000366 --> ing12_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing12_Cyanocobalamine -.-> CHEBI_17439
    ing13_Soil["Soil"]
    CultureMech_000366 --> ing13_Soil

Ingredients (14)

Preparation

1. To make up to 1 litre with distilled water. First disperse trace elements in 800ml distilled water. Add the other stock solutions and make up to 1 litre. Adjust pH to 6.8 with 1M NaOH or 1M HCl, prior to autoclaving. Autoclave at 15 psi for 15 minutes.
2. Autoclave at 15 psi for 15 minutes.
3. Ensure elements are added in the following sequence:
4. Once elements are dissolved autoclave at 15 psi for 15 minutes.
5. Filter sterile
6. Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.
7. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
8. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
9. Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.

Target organisms

No target organisms are recorded.

Provenance