modified_provasoli

CultureMech:000367 bacterial DEFINED SOLID_AGAR
Original name: Modified Provasoli

Overview

External ontology
mediadive.medium:C56 (source link unavailable) — Modified Provasoli
Applications
  • Microbial cultivation
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_MP_Mod_Provasoli.pdf

Composition

flowchart LR
    CultureMech_000367["modified_provasoli"]
    ing0_Na2_beta_glycerol_PO4_x_5_H2O["Na2 beta-glycerol PO4 x 5 H2O"]
    CultureMech_000367 --> ing0_Na2_beta_glycerol_PO4_x_5_H2O
    ing1_NaNO3["NaNO3"]
    CultureMech_000367 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_Vitamin_B12["Vitamin B12"]
    CultureMech_000367 --> ing2_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing2_Vitamin_B12 -.-> CHEBI_176843
    ing3_Thiamine["Thiamine"]
    CultureMech_000367 --> ing3_Thiamine
    CHEBI_18385["CHEBI:18385"]
    ing3_Thiamine -.-> CHEBI_18385
    ing4_Biotin["Biotin"]
    CultureMech_000367 --> ing4_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing4_Biotin -.-> CHEBI_15956
    ing5_Agar["Agar"]
    CultureMech_000367 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_000367 --> ing6_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing6_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_000367 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_H3BO3["H3BO3"]
    CultureMech_000367 --> ing8_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing8_H3BO3 -.-> CHEBI_33118
    ing9_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
    CultureMech_000367 --> ing9_MnSO4_x_4_H2O
    CHEBI_86358["CHEBI:86358"]
    ing9_MnSO4_x_4_H2O -.-> CHEBI_86358
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000367 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_000367 --> ing11_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing11_CoSO4_x_7_H2O -.-> CHEBI_91244

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Na2 beta-glycerol PO4 x 5 H2O 8 G_PER_L
2 NaNO3
NO3.Na 84.994 g/mol
110 G_PER_L CHEBI:63005
sodium nitrate
3 Vitamin B12 3.5 G_PER_L CHEBI:176843
vitamin B12
4 Thiamine
C12H17N4OS 265.362 g/mol
8 G_PER_L CHEBI:18385
thiamine(1+)
5 Biotin
C10H16N2O3S 244.316 g/mol
8 G_PER_L CHEBI:15956
biotin
6 Agar 15 G_PER_L CHEBI:2509
agar
 (for solid medium)
7 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.7 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
1.6 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
 [Merged 2 duplicates: 0.6, 1.0]
9 H3BO3
H3BO3 61.833 g/mol
1.12 G_PER_L CHEBI:33118
boric acid
10 MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
0.12 G_PER_L CHEBI:86358
manganese(II) sulfate tetrahydrate
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.022 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.005 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate

Preparation

  1. Prepare the following primary stocks solutions first.
  2. To prepare the final stock solution, Using the primary stocks as shown below, make up to 1 litre of deionised water.
  3. Dispense the final stock solution into 10ml aliquots. Autoclave at 15 psi for 15 minutes.
  4. Finally, to use add 20 ml per litre to sterile 30 ppt filtered seawater (950mls filtered seawater: 30mls deionised water)
  5. For 1/2 strength Modified Provasoli add 10 ml sterile stock per litre of sterile 30 ppt filtered seawater (950mls filtered seawater: 40mls deionised water)
  6. For agar add 15g per litre Bacteriological Agar.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.668159Z mediadive-import Imported from MediaDive Source: CCAP, ID: C56
2026-01-28T19:48:20.337639Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:03.193333Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:54.800562Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000367
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.806669+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Provasoli" to "modified_provasoli" for consistent matching
2026-06-05T07:41:37.632990+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.912184+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.