ncl_pj_0_01_npa

CultureMech:000379 bacterial COMPLEX SOLID_AGAR
Original name: NCL:PJ/0.01%NPA

Overview

External ontology
mediadive.medium:C67 (source link unavailable) — NCL:PJ/0.01%NPA
Applications
  • Microbial cultivation
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_NCL_PJ_0_01_NPA.pdf

Composition

flowchart LR
    CultureMech_000379["ncl_pj_0_01_npa"]
    ing0_Cereal_leaves["Cereal leaves"]
    CultureMech_000379 --> ing0_Cereal_leaves
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000379 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000379 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000379 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KCl["KCl"]
    CultureMech_000379 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Agar["Agar"]
    CultureMech_000379 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Cereal leaves 1.1 G_PER_L [Merged 2 duplicates: 1.0, 0.1]
2 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
4.3 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 KCl
Cl.K 74.551 g/mol
1.6 G_PER_L CHEBI:32588
potassium chloride
6 Agar 15 G_PER_L CHEBI:2509
agar

Preparation

  1. Biphasic (see separate recipes below)
  2. 3:1 mixture
  3. See separate recipes. Autoclave separately. Mix aseptically when cool.
  4. Bring PJ (Mail sol. C 76) to the boil then add cereal leaves. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes.
  5. Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.
  6. Make up 1 litre of New Cereal Leaf-­Prescott Liquid (NCL ­- see separate recipe), but use only 0.1 g of cereal leaves. Mix with 15 g of Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.
  7. Bring PJ (Mail sol. C 76) to the boil then add cereal leaves. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.707665Z mediadive-import Imported from MediaDive Source: CCAP, ID: C67
2026-03-13T03:09:29.287330Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:18:54.862030Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000379
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:05.870984+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NCL:PJ/0.01%NPA" to "ncl_pj_0_01_npa" for consistent matching
2026-06-05T07:41:39.563999+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.512325+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.