Overview
- External ontology
- mediadive.medium:C86 (source link unavailable) — RPL:PJ/0.01%RPA
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_RPL_PJ_0_01_RPA.pdf
Composition
flowchart LR
CultureMech_000400["rpl_pj_0_01_rpa"]
ing0_Rye_grass["Rye grass"]
CultureMech_000400 --> ing0_Rye_grass
ing1_K2HPO4["K2HPO4"]
CultureMech_000400 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000400 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000400 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_000400 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_Agar["Agar"]
CultureMech_000400 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Rye grass | 1.1 G_PER_L | [Merged 2 duplicates: 1.0, 0.1] | |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
1.6 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Biphasic (see separate recipes below and overleaf)
- 3:1 mixture.
- See separate recipes. Autoclave separately. Mix aseptically when cool.
- Bring PJ (Mail sol. C 76) to the boil then add rye grass. Continue to boil for 5 minutes. Allow to cool then restore volume to 1 litre with deionized water. Filter through GF/C paper and autoclave at 15 psi for 15 minutes.
- Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.
- Make up 1 litre of Rye GrassPrescott Liquid (RPL - see separate recipe), but use only 0.1 g of rye grass. Mix with 15 g of Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.762518Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C86 |
| 2026-03-13T03:08:46.223412Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:54.952513Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000400 |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:05.966020+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RPL:PJ/0.01%RPA" to "rpl_pj_0_01_rpa" for consistent matching |
| 2026-06-05T07:41:44.707629+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.095929+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |