se2

CultureMech:000406 bacterial COMPLEX LIQUID
Original name: SE2

Overview

External ontology
mediadive.medium:C91 (source link unavailable) — SE2
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_SE2_Freshwater.pdf CCAP prepares SE2 by twice autoclaving dried calcareous garden loam with twice its volume of deionized water, settling it for 2-3 weeks, decanting the supernatant, and autoclaving the final extract.

Composition

flowchart LR
    CultureMech_000406["se2"]
    ing0_Prepared_dried_calcareous_garden_loam["Prepared dried calcareous garden loam"]
    CultureMech_000406 --> ing0_Prepared_dried_calcareous_garden_loam
    ing1_Deionized_water["Deionized water"]
    CultureMech_000406 --> ing1_Deionized_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Deionized_water -.-> CHEBI_15377

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Prepared dried calcareous garden loam variable VARIABLE ENVO:00001998
soil
 CCAP specifies air-dried and sieved calcareous garden loam.
2 Deionized water
H2O 18.015 g/mol
2 volumes per 1 volume dried soil VARIABLE CHEBI:15377
water
 CCAP mixes dried soil with twice its volume of deionized water.

Preparation

  1. Air-dry calcareous garden loam, remove debris, and sieve it.
  2. Combine dried soil with twice its volume of deionized water.
  3. Autoclave at 15 psi and 126 C for 20 minutes, cool, and repeat once.
  4. Seal and leave undisturbed for 2-3 weeks to settle.
  5. Aseptically decant the supernatant into sterile containers.
  6. Autoclave the final soil extract at 15 psi and 121 C for 15 minutes.
  7. Cool, seal, and store refrigerated.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.780323Z mediadive-import Imported from MediaDive Source: CCAP, ID: C91
2026-03-13T06:18:54.994192Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000406
2026-03-17T19:45:06.004460+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SE2" to "se2" for consistent matching
2026-08-25T00:00:00-07:00 repair_ccap_duplicate_compositions.py SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE Rebuilt se2 from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM.
2026-09-07T00:00:00-07:00 repair_bacterial_se2_score35.py GROUNDED_BACTERIAL_CCAP_SE2_SCORE35 Aligned the bacterial CCAP SE2 duplicate with the reviewed two-component SE2 source recipe.