Overview
- External ontology
- mediadive.medium:C92 (source link unavailable) — SES
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_SES.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_SES.pdf on 2026-08-25.
Composition
flowchart LR
CultureMech_000407["ses"]
ing0_Deionized_water["Deionized water"]
CultureMech_000407 --> ing0_Deionized_water
CHEBI_15377["CHEBI:15377"]
ing0_Deionized_water -.-> CHEBI_15377
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Deionized water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Add the four stock solutions and make up to 1 L with deionized water.
- Autoclave at 15 psi for 15 minutes.
Solutions
K2HPO4 stock solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| K2HPO4 | 1.0 G_PER_L |
CCAP SES stock solution 1; add 20 ml/L.
MgSO4.7H2O stock solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4.7H2O | 1.0 G_PER_L |
CCAP SES stock solution 2; add 20 ml/L.
KNO3 stock solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KNO3 | 10.0 G_PER_L |
CCAP SES stock solution 3; add 20 ml/L.
Soil Extract 2 (SE2)
Dose: 100.0 ML_PER_L
Use the separately defined CCAP SE2 recipe.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.782374Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C92 |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Soil |
| 2026-03-13T06:18:54.998478Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000407 |
| 2026-03-17T06:29:46.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.009694+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SES" to "ses" for consistent matching |
| 2026-05-16T09:04:31.651939+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:45.769898+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.835775+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:11.463688+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-25T00:00:00-07:00 | repair_ccap_duplicate_compositions.py | SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE | Rebuilt ses from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM. |