ses_mp

Overview

External ontology

mediadive.medium:C93 (source link unavailable) — SES:MP

Applications

• Microbial cultivation

Notes

Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_SES_MP.pdf

Composition

flowchart LR
    CultureMech_000408["ses_mp"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_000408 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000408 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_KNO3["KNO3"]
    CultureMech_000408 --> ing2_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing2_KNO3 -.-> CHEBI_63043
    ing3_Soil["Soil"]
    CultureMech_000408 --> ing3_Soil
    ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
    CultureMech_000408 --> ing4_Ca_NO3_2_x_4_H2O
    CHEBI_86159["CHEBI:86159"]
    ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
    ing5_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_000408 --> ing5_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing5_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing6_KCl["KCl"]
    CultureMech_000408 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_Fe_SO4_x_7_H2O["Fe SO4 x 7 H2O"]
    CultureMech_000408 --> ing7_Fe_SO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_Fe_SO4_x_7_H2O -.-> CHEBI_75836
    ing8_H2SO4["H2SO4"]
    CultureMech_000408 --> ing8_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing8_H2SO4 -.-> CHEBI_26836

Ingredients (9)

Preparation

1. 3:1 mixture
2. See separate recipes. Autoclave separately. Mix aseptically.
3. Make up to 1 litre with deionized water and autoclave at 15 psi for 15 minutes.
4. At the CCAP, SE1 is used for marine algae, SE2 for freshwater and terrestrial protozoa.
5. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
6. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
7. Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.
8. Site selection for a good soil is very important and for most purposes a soil from undisturbed deciduous woodland is best. Sites to avoid are those showing obvious signs of man's activity and particular care should be taken to avoid areas where fertilizers, crop sprays or other toxic chemicals may have been used.
9. A rich loam with good crumb structure should be sought. Stones, roots and larger invertebrates should be removed during an initial sieving through a 1 cm mesh. The sieved soil should be spread to air dry and handpicked for smaller invertebrates and roots. It should be turned periodically and picked over again. When dry sieve through a finer mesh (2-4 mm) and store in an airtight container away from light and heat.
10. Soil is prepared as above. Air-dried soil and twice its volume of supernatant distilled water are autoclaved together at 15 psi for 2 hours and left to cool. The supernatant is then decanted and then distributed to containers in volumes suitable for making up batches of media. The aliquots and their containers are autoclaved for an appropriate length of time (e.g. 1 litre or less for 15 minutes) and are then kept in a cool place (e.g. a refrigerator) until required.
11. Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance