Overview
- External ontology
- mediadive.medium:C96 (source link unavailable) — 2SNA
- Applications
-
- Microbial cultivation
- Notes
- Source: CCAP | Link: https://www-cyanosite.bio.purdue.edu/media/table/SNA.html Composition verified against https://www-cyanosite.bio.purdue.edu/media/table/SNA.html on 2026-08-25.
Composition
flowchart LR
CultureMech_000411["2sna"]
ing0_Nutrient_agar__Oxoid_CM3_["Nutrient agar (Oxoid CM3)"]
CultureMech_000411 --> ing0_Nutrient_agar__Oxoid_CM3_
ing1_NaCl["NaCl"]
CultureMech_000411 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Filtered_natural_seawater["Filtered natural seawater"]
CultureMech_000411 --> ing2_Filtered_natural_seawater
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Nutrient agar (Oxoid CM3) | 28.0 G_PER_L | CCAP 2SNA lists 28.0 g Nutrient agar (Oxoid CM3). | |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
35.0 G_PER_L |
CHEBI:26710
sodium chloride
|
CCAP 2SNA lists 35.0 g NaCl. |
| 3 | Filtered natural seawater | 1000 ML_PER_L | CCAP 2SNA lists 1000 mL filtered natural seawater. The source also permits 35 g/L synthetic sea salts in distilled water. |
Preparation
- Mix the constituents and ensure homogeneity.
- Steam for 30 minutes.
- Dispense as required.
- Autoclave at 15 psi.
- For tubes, slope before the medium cools and solidifies.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.797266Z | mediadive-import | Imported from MediaDive | Source: CCAP, ID: C96 |
| 2026-02-03T11:26:44.517650 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:55.023791Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000411 |
| 2026-03-17T19:45:06.040258+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "2SNA" to "2sna" for consistent matching |
| 2026-08-06T07:56:36.078058+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_ccap_missing_compositions.py | RESTORED_CCAP_SOURCE_COMPOSITION | Restored 2sna from the reviewed authoritative source. Preserved stock boundaries and source alternatives; removed the incomplete flag where present. Ingredient identities follow the MediaIngredientMech SSSOM. |
| 2026-09-11T00:00:00-07:00 | repair_ccap_2sna_score15.py | RESOLVED_CCAP_2SNA_SCORE15 | CCAP 2SNA lists 28.0 g Nutrient agar (Oxoid CM3), 35.0 g NaCl, and 1000 mL filtered natural seawater. |