sp

CultureMech:000413 bacterial DEFINED LIQUID
Original name: SP

Overview

External ontology
mediadive.medium:C98 (source link unavailable) — SP
Applications
  • Microbial cultivation
Notes
Source: CCAP | Link: https://www.ccap.ac.uk/wp-content/uploads/MR_SP.pdf

Composition

flowchart LR
    CultureMech_000413["sp"]
    ing0_NaHCO3["NaHCO3"]
    CultureMech_000413 --> ing0_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing0_NaHCO3 -.-> CHEBI_32139
    ing1_Na2CO3["Na2CO3"]
    CultureMech_000413 --> ing1_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing1_Na2CO3 -.-> CHEBI_29377
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000413 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_NaNO3["NaNO3"]
    CultureMech_000413 --> ing3_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing3_NaNO3 -.-> CHEBI_63005
    ing4_K2SO4["K2SO4"]
    CultureMech_000413 --> ing4_K2SO4
    CHEBI_32036["CHEBI:32036"]
    ing4_K2SO4 -.-> CHEBI_32036
    ing5_NaCl["NaCl"]
    CultureMech_000413 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000413 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000413 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000413 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_Na2_EDTA["Na2-EDTA"]
    CultureMech_000413 --> ing9_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na2_EDTA -.-> CHEBI_64734
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000413 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnSO4_x_7_H2O["MnSO4 x 7 H2O"]
    CultureMech_000413 --> ing11_MnSO4_x_7_H2O
    CHEBI_86360["CHEBI:86360"]
    ing11_MnSO4_x_7_H2O -.-> CHEBI_86360
    ing12_H3BO3["H3BO3"]
    CultureMech_000413 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000413 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
    CultureMech_000413 --> ing14_Co_NO3_2_x_6_H2O
    CHEBI_86214["CHEBI:86214"]
    ing14_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
    ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000413 --> ing15_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing16_Biotin["Biotin"]
    CultureMech_000413 --> ing16_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing16_Biotin -.-> CHEBI_15956
    ing17_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000413 --> ing17_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_pantothenate -.-> CHEBI_31345
    ing18_Cyanocobalamine["Cyanocobalamine"]
    CultureMech_000413 --> ing18_Cyanocobalamine
    CHEBI_17439["CHEBI:17439"]
    ing18_Cyanocobalamine -.-> CHEBI_17439
    ing19_Folic_acid["Folic acid"]
    CultureMech_000413 --> ing19_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing19_Folic_acid -.-> CHEBI_27470
    ing20_Inositol["Inositol"]
    CultureMech_000413 --> ing20_Inositol
    CHEBI_24848["CHEBI:24848"]
    ing20_Inositol -.-> CHEBI_24848
    ing21_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000413 --> ing21_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing21_Nicotinic_acid -.-> CHEBI_15940
    ing22_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000413 --> ing22_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing22_Thiamine_HCl -.-> CHEBI_49105
    ing23_Thymine["Thymine"]
    CultureMech_000413 --> ing23_Thymine
    CHEBI_17821["CHEBI:17821"]
    ing23_Thymine -.-> CHEBI_17821

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 NaHCO3
CHO3.Na 84.006 g/mol
27.22 G_PER_L CHEBI:32139
sodium hydrogencarbonate
2 Na2CO3
CO3.2Na 105.988 g/mol
8.06 G_PER_L CHEBI:29377
sodium carbonate
3 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 NaNO3
NO3.Na 84.994 g/mol
5 G_PER_L CHEBI:63005
sodium nitrate
5 K2SO4
2K.O4S 174.259 g/mol
2 G_PER_L CHEBI:32036
potassium sulfate
6 NaCl
Cl.Na 58.443 g/mol
2 G_PER_L CHEBI:26710
sodium chloride
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.72 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 [Merged 2 duplicates: 0.02, 0.7]
10 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.9600000000000001 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
 [Merged 2 duplicates: 0.16, 0.8]
11 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.001 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
12 MnSO4 x 7 H2O
Mn.O4S 151.001 g/mol
0.002 G_PER_L CHEBI:86360
manganese(II) sulfate
13 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.001 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
0.001 G_PER_L CHEBI:86214
cobalt dinitrate hexahydrate
16 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
5e-05 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
17 Biotin
C10H16N2O3S 244.316 g/mol
0.0002 G_PER_L CHEBI:15956
biotin
18 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.02 G_PER_L CHEBI:31345
Calcium pantothenate
19 Cyanocobalamine
C63H88CoN14O14P 1355.388 g/mol
0.004 G_PER_L CHEBI:17439
cyanocob(III)alamin
20 Folic acid
C19H19N7O6 441.404 g/mol
0.0004 G_PER_L CHEBI:27470
folic acid
21 Inositol
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:24848
inositol
22 Nicotinic acid
C6H5NO2 123.111 g/mol
0.02 G_PER_L CHEBI:15940
nicotinic acid
23 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.1 G_PER_L CHEBI:49105
thiamine hydrochloride
24 Thymine
C5H6N2O2 126.115 g/mol
0.6 G_PER_L CHEBI:17821
thymine

Preparation

  1. This medium is made up in 2 parts to reduce precipitation when autoclaving. For each part add the components to around 900 mls deionised water and then top up to 1 litre with deionised water.
  2. Autoclave parts 1 and 2 separately at 15 psi, allow to cool then mix 1:1 aseptically.
  3. May be stored frozen at -20 °C.
  4. May be stored frozen at -20 °C.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:19.801318Z mediadive-import Imported from MediaDive Source: CCAP, ID: C98
2026-03-13T03:09:30.197016Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:18:55.034930Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000413
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.052106+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SP" to "sp" for consistent matching
2026-06-05T07:41:46.103583+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.565627+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.