Overview
- External ontology
- mediadive.medium:1003 (source link unavailable) — HYDROGEN-OXYDIZING MEDIUM
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1003.pdf
Composition
flowchart LR
CultureMech_000419["hydrogen_oxydizing_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000419 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_NaS2O3["NaS2O3"]
CultureMech_000419 --> ing1_NaS2O3
CHEBI_132112["CHEBI:132112"]
ing1_NaS2O3 -.-> CHEBI_132112
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000419 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_000419 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_MgCl2["MgCl2"]
CultureMech_000419 --> ing4_MgCl2
CHEBI_6636["CHEBI:6636"]
ing4_MgCl2 -.-> CHEBI_6636
ing5_MES["MES"]
CultureMech_000419 --> ing5_MES
CHEBI_39010["CHEBI:39010"]
ing5_MES -.-> CHEBI_39010
ing6_NH4Cl["NH4Cl"]
CultureMech_000419 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_MgCl2_x_H2O["MgCl2 x H2O"]
CultureMech_000419 --> ing7_MgCl2_x_H2O
CHEBI_86355["CHEBI:86355"]
ing7_MgCl2_x_H2O -.-> CHEBI_86355
ing8_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_000419 --> ing8_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing8_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing9_Na_EDTA_x_2_H2O["Na-EDTA x 2 H2O"]
CultureMech_000419 --> ing9_Na_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing9_Na_EDTA_x_2_H2O -.-> CHEBI_64758
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000419 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000419 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000419 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_ZnCl2["ZnCl2"]
CultureMech_000419 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
CultureMech_000419 --> ing14_AlCl3_x_6_H2O
CHEBI_30115["CHEBI:30115"]
ing14_AlCl3_x_6_H2O -.-> CHEBI_30115
ing15_Na_tungstate_x_2_H2O["Na-tungstate x 2 H2O"]
CultureMech_000419 --> ing15_Na_tungstate_x_2_H2O
ing16_CuCl["CuCl"]
CultureMech_000419 --> ing16_CuCl
CHEBI_53472["CHEBI:53472"]
ing16_CuCl -.-> CHEBI_53472
ing17_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
CultureMech_000419 --> ing17_NiSO4_x_6_H2O
CHEBI_53001["CHEBI:53001"]
ing17_NiSO4_x_6_H2O -.-> CHEBI_53001
ing18_Se_acid["Se-acid"]
CultureMech_000419 --> ing18_Se_acid
ing19_HBO3["HBO3"]
CultureMech_000419 --> ing19_HBO3
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000419 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
NaS2O3
2Na.O3S2 158.111 g/mol
|
2 G_PER_L |
CHEBI:132112
sodium thiosulfate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
40.4 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.4, 40.0] |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.48 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
MgCl2
2Cl.Mg 95.211 g/mol
|
0.78 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 6 | MES | 1.95 G_PER_L |
CHEBI:39010
MES
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
100 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
MgCl2 x H2O
2Cl.H2O.Mg 113.226 g/mol
|
100 G_PER_L |
CHEBI:86355
magnesium dichloride monohydrate
|
|
| 9 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
200 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 10 |
Na-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
0.5 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.15 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 |
AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
|
0.04 G_PER_L |
CHEBI:30115
aluminium trichloride hexahydrate
|
|
| 16 | Na-tungstate x 2 H2O | 0.03 G_PER_L | ||
| 17 |
CuCl
ClCu 98.999 g/mol
|
0.02 G_PER_L |
CHEBI:53472
copper(I) chloride
|
|
| 18 |
NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
|
0.02 G_PER_L |
CHEBI:53001
nickel sulfate
|
|
| 19 | Se-acid | 0.01 G_PER_L | ||
| 20 | HBO3 | 0.01 G_PER_L | ||
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Place 1000 ml of anaerobic water (CO2 water) in a flask. Add the following compounds:
- Add solutions A, B, and Trace mineral solution (see recipes below):
- pH the media to 6.0 and gas the flask containing the media with CO2 for 20 min at least. Gas first culture tube in rack and place 5 ml of media in previous tube. Place stopper on loaded culture tube, crimp tube cap onto stopper. Autoclave the medium for 20 min., 121°C. Add 1 ml of O2 to each tube before inoculation. After the inoculation the tubes are pressurized with H2 (138 KP).
- Adjust the pH to 4 with HCl.
- Adjust the pH of the solution to 3.0 with HCl.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.052815Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1003 |
| 2026-01-28T19:48:20.090560Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:09:02.748784Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:55.091582Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000419 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.115510+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYDROGEN-OXYDIZING MEDIUM" to "hydrogen_oxydizing_medium" for consistent matching |
| 2026-06-05T07:41:08.883037+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.864101+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |