Overview
- External ontology
- mediadive.medium:1006 (source link unavailable) — ALLISONELLA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1006.pdf Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The original recipe adds carbonate, fatty-acid stock and cysteine after cooling under CO2, then dispenses and autoclaves. The API main volume of 1004 ml is a provider encoding, not evidence of measured final volume or verified density conversions. Source-specific ingredient identities and quantity scopes supersede copied flattened amounts. Unknown gas amounts, densities and final volumes remain unknown. Other existing chemical annotations and all prior history are preserved, not recertified. No taxa-media association is added.
Composition
flowchart LR
CultureMech_000422["allisonella_medium"]
ing0_DL_Histidine["DL-Histidine"]
CultureMech_000422 --> ing0_DL_Histidine
CHEBI_27570["CHEBI:27570"]
ing0_DL_Histidine -.-> CHEBI_27570
ing1_Yeast_extract["Yeast extract"]
CultureMech_000422 --> ing1_Yeast_extract
ing2_Trypticase_peptone["Trypticase peptone"]
CultureMech_000422 --> ing2_Trypticase_peptone
ing3_K2HPO4["K2HPO4"]
CultureMech_000422 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_KH2PO4["KH2PO4"]
CultureMech_000422 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_000422 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_NaCl["NaCl"]
CultureMech_000422 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000422 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000422 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_Na2CO3["Na2CO3"]
CultureMech_000422 --> ing9_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing9_Na2CO3 -.-> CHEBI_29377
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000422 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Distilled_water["Distilled water"]
CultureMech_000422 --> ing11_Distilled_water
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
DL-Histidine
C6H9N3O2 155.157 g/mol
|
CHEBI:27570
histidine
|
Source batch input: 7.8 g. Preserve the exact mass in notes; API g_l=7.76892 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | |
| 2 | Yeast extract | Source batch input: 4 g. Preserve the exact mass in notes; API g_l=3.98406 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | ||
| 3 | Trypticase peptone | Source batch input: 1 g. Preserve the exact mass in notes; API g_l=0.996016 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. Source material qualifier: BD BBL. | ||
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
CHEBI:131527
dipotassium hydrogen phosphate
|
Source batch input: 0.29 g. Preserve the exact mass in notes; API g_l=0.288845 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
CHEBI:63036
potassium dihydrogen phosphate
|
Source batch input: 0.29 g. Preserve the exact mass in notes; API g_l=0.288845 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
CHEBI:62946
ammonium sulfate
|
Source batch input: 0.48 g. Preserve the exact mass in notes; API g_l=0.478088 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
CHEBI:26710
sodium chloride
|
Source batch input: 0.48 g. Preserve the exact mass in notes; API g_l=0.478088 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | |
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
CHEBI:31795
magnesium sulfate heptahydrate
|
Source batch input: 0.1 g. Preserve the exact mass in notes; API g_l=0.0996016 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | |
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
CHEBI:86158
calcium chloride dihydrate
|
Source batch input: 64 mg. Preserve the exact mass in notes; API g_l=0.063745 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | |
| 10 |
Na2CO3
CO3.2Na 105.988 g/mol
|
CHEBI:29377
sodium carbonate
|
Source batch input: 4 g. Preserve the exact mass in notes; API g_l=3.98406 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | |
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Source batch input: 0.5 g. Preserve the exact mass in notes; API g_l=0.498008 uses the provider's main-volume encoding and is not an independently verified final-medium concentration. | |
| 12 | Distilled water | 1 L | Source input: 1000 ml = 1 L distilled water, not a measured final-volume denominator after the other additions. |
Preparation
- Dissolve ingredients (except carbonate, fatty acid mixture and cysteine), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate, fatty acid mixture and cysteine and adjust pH to 6.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.0, if necessary.
Solutions
Sodium resazurin (0.1% w/v)
Dose: 0.0005 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Sodium resazurin | 0.1 PERCENT_W_V | The explicitly stated 0.1% w/v belongs to the sodium resazurin stock. Its 0.50 ml receiving-medium dose is stored separately; no final-medium concentration is inferred. |
Source addition: 0.50 ml = 0.0005 L of 0.1% w/v sodium resazurin stock, not a mass or final-medium concentration.
Volatile fatty acid mixture
Dose: 0.0031 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Acetic acid | 0.5485 L | Stock preparation input: 548.5 ml = 0.5485 L. This is a liquid input volume, not grams or a finished-medium concentration. Source API g_l/mmol_l fields are not adopted because their density and conversion basis are not independently established by the original recipe. |
| Propionic acid | 0.1935 L | Stock preparation input: 193.5 ml = 0.1935 L. This is a liquid input volume, not grams or a finished-medium concentration. Source API g_l/mmol_l fields are not adopted because their density and conversion basis are not independently established by the original recipe. |
| Butyric acid | 0.129 L | Stock preparation input: 129 ml = 0.129 L. This is a liquid input volume, not grams or a finished-medium concentration. Source API g_l/mmol_l fields are not adopted because their density and conversion basis are not independently established by the original recipe. |
| n-Valeric acid | 0.03225 L | Stock preparation input: 32.25 ml = 0.03225 L. This is a liquid input volume, not grams or a finished-medium concentration. Source API g_l/mmol_l fields are not adopted because their density and conversion basis are not independently established by the original recipe. |
| iso-Butyric acid | 0.03225 L | Stock preparation input: 32.25 ml = 0.03225 L. This is a liquid input volume, not grams or a finished-medium concentration. Source API g_l/mmol_l fields are not adopted because their density and conversion basis are not independently established by the original recipe. |
| DL-2-Methylbutyric acid | 0.03225 L | Stock preparation input: 32.25 ml = 0.03225 L. This is a liquid input volume, not grams or a finished-medium concentration. Source API g_l/mmol_l fields are not adopted because their density and conversion basis are not independently established by the original recipe. |
| iso-Valeric acid | 0.03225 L | Stock preparation input: 32.25 ml = 0.03225 L. This is a liquid input volume, not grams or a finished-medium concentration. Source API g_l/mmol_l fields are not adopted because their density and conversion basis are not independently established by the original recipe. |
Source addition: 3.10 ml = 0.0031 L fatty-acid mixture. The nested acid volumes prepare that stock; they are not added individually in those volumes to the receiving medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:13.066244Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1006 |
| 2026-03-13T06:18:55.131266Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000422 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.158715+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALLISONELLA MEDIUM" to "allisonella_medium" for consistent matching |
| 2026-06-05T07:40:48.260178+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.937163+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T02:20:31.460987+00:00 | codex | RESTORED_ALLISONELLA_ACID_IDENTITIES_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore source-specific Allisonella ingredients and the acid/resazurin stock boundaries; retain unquantified amounts, chemical-form limits and source conflicts, and correct the reciprocal family relationship without changing permanent IDs. |