mineral_medium

CultureMech:000423 bacterial DEFINED LIQUID
Original name: MINERAL MEDIUM

Overview

External ontology
mediadive.medium:1007 (source link unavailable) — MINERAL MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 5.5, 'max': 6.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1007.pdf

Composition

flowchart LR
    CultureMech_000423["mineral_medium"]
    ing0_KNO3["KNO3"]
    CultureMech_000423 --> ing0_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing0_KNO3 -.-> CHEBI_63043
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000423 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000423 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000423 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Distilled_water["Distilled water"]
    CultureMech_000423 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 KNO3
K.NO3 101.102 g/mol
0.25 G_PER_L CHEBI:63043
potassium nitrate
 DSMZ Medium 1007 lists 250.0 mg KNO3 in the main 1000 ml solution.
2 KH2PO4
H2O4P.K 136.084 g/mol
0.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 DSMZ Medium 1007 lists 100.0 mg KH2PO4 in the main 1000 ml solution.
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 DSMZ Medium 1007 lists 50.0 mg MgSO4 x 7 H2O in the main 1000 ml solution.
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.01 G_PER_L CHEBI:86158
calcium chloride dihydrate
 DSMZ Medium 1007 lists 10.0 mg CaCl2 x 2 H2O in the main 1000 ml solution.
5 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 DSMZ Medium 1007 makes the main solution with 1000.0 ml distilled water.

Preparation

  1. Adjust the final medium to pH 5.5-6.0.
  2. Use shaking incubation for strains grown on methane.

Solutions

Trace elements

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
EDTA 5.00 G_PER_L DSMZ Medium 1007 lists 5.00 g EDTA in the trace elements stock.
CuCl2 x 5 H2O 0.10 G_PER_L DSMZ Medium 1007 lists 0.10 g CuCl2 x 5 H2O in the trace elements stock.
FeSO4 x 7 H2O 2.00 G_PER_L DSMZ Medium 1007 lists 2.00 g FeSO4 x 7 H2O in the trace elements stock.
ZnSO4 x 7 H2O 0.10 G_PER_L DSMZ Medium 1007 lists 0.10 g ZnSO4 x 7 H2O in the trace elements stock.
NiCl2 x 6 H2O 0.02 G_PER_L DSMZ Medium 1007 lists 0.02 g NiCl2 x 6 H2O in the trace elements stock.
CoCl2 x 6 H2O 0.20 G_PER_L DSMZ Medium 1007 lists 0.20 g CoCl2 x 6 H2O in the trace elements stock.
Na2MoO4 0.03 G_PER_L DSMZ Medium 1007 lists 0.03 g Na2MoO4 in the trace elements stock.
MnCl2 x 4 H2O 0.03 G_PER_L DSMZ Medium 1007 lists 0.03 g MnCl2 x 4 H2O in the trace elements stock.
Distilled water 1000.00 ML_PER_L DSMZ Medium 1007 makes the trace elements stock with 1000.00 ml distilled water.
DSMZ Medium 1007 adds 1.0 ml/L trace elements stock.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.069358Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1007
2026-03-13T06:18:55.138343Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000423
2026-03-17T06:29:44.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.166898+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MINERAL MEDIUM" to "mineral_medium" for consistent matching
2026-05-20T06:01:57.421358+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '5.5-6' -> {'min': 5.5, 'max': 6.0}
2026-05-24T04:38:33.207592+00:00 backfill_source_environment.py BACKFILLED_SOURCE_ENVIRONMENT added=4 env(s) from CommunityMech
2026-06-05T07:41:34.067471+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.597610+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.851204+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00 repair_dsmz_1007_trace_stock_score15.py RESOLVED_DSMZ_1007_TRACE_STOCK_SCORE15 DSMZ Medium 1007 defines Mineral Medium with KNO3, KH2PO4, MgSO4 x 7 H2O, CaCl2 x 2 H2O, 1.0 ml/L trace elements, and distilled water at final pH 5.5-6.0. The trace elements stock contains EDTA, CuCl2 x 5 H2O, FeSO4 x 7 H2O, ZnSO4 x 7 H2O, NiCl2 x 6 H2O, CoCl2 x 6 H2O, Na2MoO4, MnCl2 x 4 H2O, and distilled water per liter.