sediminispirochaeta_medium

CultureMech:000424 bacterial COMPLEX LIQUID
Original name: SEDIMINISPIROCHAETA MEDIUM

Overview

External ontology
mediadive.medium:1008 (source link unavailable) — SEDIMINISPIROCHAETA MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1008.pdf

Composition

flowchart LR
    CultureMech_000424["sediminispirochaeta_medium"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_000424 --> ing0_Trypticase_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000424 --> ing1_Yeast_extract
    ing2_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000424 --> ing2_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Sodium_resazurin -.-> CHEBI_8806
    ing3_Na_thioglycolate["Na-thioglycolate"]
    CultureMech_000424 --> ing3_Na_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing3_Na_thioglycolate -.-> CHEBI_86481
    ing4_Cellobiose["Cellobiose"]
    CultureMech_000424 --> ing4_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing4_Cellobiose -.-> CHEBI_17057
    ing5_Charcoal_filtered__natural_seawater["Charcoal-filtered, natural seawater"]
    CultureMech_000424 --> ing5_Charcoal_filtered__natural_seawater

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Trypticase peptone 2 G_PER_L
2 Yeast extract 1 G_PER_L
3 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
4 Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
1 G_PER_L CHEBI:86481
sodium thioglycolate
5 Cellobiose
C12H22O11 342.297 g/mol
2 G_PER_L CHEBI:17057
cellobiose
6 Charcoal-filtered, natural seawater 800 G_PER_L

Preparation

  1. Dissolve ingredients except thioglycolate and cellobiose, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add thioglycolate and adjust pH of medium to 7.5 with 10 N KOH. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 15 min. Add cellobiose from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration.
  2. Note: Bottled seawater from Biomaris GmbH can be used instead of filtered seawater.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.071512Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1008
2026-03-13T06:18:55.143099Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000424
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.172101+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SEDIMINISPIROCHAETA MEDIUM" to "sediminispirochaeta_medium" for consistent matching
2026-06-05T07:41:45.598669+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.405197+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T13:32:40.086487+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.