Overview
- External ontology
- mediadive.medium:1011c (source link unavailable) — DESULFOTHERMUS MJ MEDIUM (H2/CO2)
- Applications
-
- Microbial cultivation
- pH
- 6.7
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1011c.pdf
Composition
flowchart LR
CultureMech_000430["desulfothermus_mj_medium_h2_co2"]
ing0_NaCl["NaCl"]
CultureMech_000430 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_000430 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000430 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000430 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000430 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_000430 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NH4Cl["NH4Cl"]
CultureMech_000430 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_000430 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing8_Na2CO3["Na2CO3"]
CultureMech_000430 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Na_pyruvate["Na-pyruvate"]
CultureMech_000430 --> ing9_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing9_Na_pyruvate -.-> CHEBI_50144
ing10_Na_lactate["Na-lactate"]
CultureMech_000430 --> ing10_Na_lactate
CHEBI_75228["CHEBI:75228"]
ing10_Na_lactate -.-> CHEBI_75228
ing11_Yeast_extract["Yeast extract"]
CultureMech_000430 --> ing11_Yeast_extract
ing12_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_000430 --> ing12_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing12_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000430 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000430 --> ing14_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing14_MnSO4_x_H2O -.-> CHEBI_86364
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000430 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_000430 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000430 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000430 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000430 --> ing19_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing19_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing20_H3BO3["H3BO3"]
CultureMech_000430 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000430 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000430 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_000430 --> ing23_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000430 --> ing24_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing25_Biotin["Biotin"]
CultureMech_000430 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_Folic_acid["Folic acid"]
CultureMech_000430 --> ing26_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing26_Folic_acid -.-> CHEBI_27470
ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000430 --> ing27_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing28_Thiamine_HCl["Thiamine HCl"]
CultureMech_000430 --> ing28_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing28_Thiamine_HCl -.-> CHEBI_49105
ing29_Riboflavin["Riboflavin"]
CultureMech_000430 --> ing29_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing29_Riboflavin -.-> CHEBI_17015
CultureMech_000430 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
30.6443 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 29.6443, 1.0] |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.13834 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.23834 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.13834, 0.1] |
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.35968 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.35968, 3.0] |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.13043 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.326087 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.247036 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00988142 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.48221 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
0.494071 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 11 |
Na-lactate
C3H5O3.Na 112.06 g/mol
|
0.494071 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 12 | Yeast extract | 0.0988142 G_PER_L | ||
| 13 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.48221 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 25 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 27 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 28 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 29 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 30 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 31 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 32 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 33 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 34 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 35 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 36 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 37 |
Na2S2O4
2Na.O4S2 174.11 g/mol
|
50 G_PER_L |
CHEBI:66870
sodium dithionite
|
Preparation
- Dissolve ingredients (except carbonate, pyruvate, lactate, yeast extract, thiosulfate, vitamins and dithionite), then sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials up to a volume of 20% and autoclave. Add pyruvate, lactate, yeast extract, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate and dithionite from sterile anoxic stock solutions prepared under 80% N2 and 20% CO2 gas mixture. Solutions of vitamins, thiosulfate and dithionite are sterilized by filtration. Adjust pH of the complete medium to 6.7.
- After inoculation pressurize vessels to 2 bar overpressure with sterile 80% H2 and 20% CO2 gas mixture.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
- Add 1 N NaOH to distilled water and make the solution anoxic by sparging with 100% N2 gas. Then dissolve the Na-dithionite and filter-sterilize the solution into anoxic Hungate tubes. The final pH of the solution should be between 9 and 10. Store the prepared solution in a dark and cool place. Prepare only small amounts of stock solution, as Na-dithionite decomposes rapidly.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.094239Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1011c |
| 2026-01-28T19:48:09.396118Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-06T05:10:15.036319Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:42.378345Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:18:55.222732Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000430 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.258655+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOTHERMUS MJ MEDIUM (H2/CO2)" to "desulfothermus_mj_medium_h2_co2" for consistent matching |
| 2026-06-05T07:40:57.425511+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.886246+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |