Overview
- External ontology
- mediadive.medium:1012 (source link unavailable) — PEREDIBACTER (DN) MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1012.pdf
Composition
flowchart LR
CultureMech_000432["peredibacter_dn_medium"]
ing0_Nutrient_broth["Nutrient broth"]
CultureMech_000432 --> ing0_Nutrient_broth
ing1_Casamino_acids["Casamino acids"]
CultureMech_000432 --> ing1_Casamino_acids
ing2_Yeast_extract["Yeast extract"]
CultureMech_000432 --> ing2_Yeast_extract
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000432 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000432 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Nutrient broth | 0.8 G_PER_L | ||
| 2 | Casamino acids | 0.5 G_PER_L |
FOODON:03315719
Casamino acids
|
|
| 3 | Yeast extract | 0.1 G_PER_L |
FOODON:03315426
Yeast extract
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.6 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Preparation
- Dissolve ingredients except calcium and magnesium chloride and adjust pH of the medium to 7.2 with NaOH. After autoclaving add calcium and magnesium from sterile stock solutions.
- Before inoculation prepare a suspension of prey cells in 30-40 ml medium using a 250 ml flask. Usually the resuspension of two overnight cultures of the prey strain on agar slants of DSMZ medium 54 will yield a final concentration of 10<sup>8</sup> to 10<sup>9</sup> cells per ml culture broth.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.104924Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1012 |
| 2026-01-28T19:48:14.981849Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:18:55.247533Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000432 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.286880+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PEREDIBACTER (DN) MEDIUM" to "peredibacter_dn_medium" for consistent matching |
| 2026-06-05T07:41:41.339475+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.627382+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-09-13T00:00:00-07:00 | repair_bacterial_score10_exact_terms_batch6.py | GROUNDED_BACTERIAL_SCORE10_EXACT_TERMS_BATCH6 | Applied local mappings for Casamino acids, Yeast extract. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |