bdellovibrio_bacteriovorus_medium

Overview

External ontology

mediadive.medium:1012a (source link unavailable) — BDELLOVIBRIO BACTERIOVORUS MEDIUM

Applications

• Microbial cultivation

pH

7.2

Notes

Source: DSMZ

Composition

flowchart LR
    CultureMech_000433["bdellovibrio_bacteriovorus_medium"]
    ing0_Nutrient_broth["Nutrient broth"]
    CultureMech_000433 --> ing0_Nutrient_broth
    ing1_Casamino_acids["Casamino acids"]
    CultureMech_000433 --> ing1_Casamino_acids
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000433 --> ing2_Yeast_extract
    ing3_Agar["Agar"]
    CultureMech_000433 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000433 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000433 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158

Ingredients (6)

Preparation

1. Preparation of double-layered agar plates:<p> <b>Bottom layer:</b> Dissolve ingredients and adjust the pH of the medium to 7.2 with NaOH. Then bring the medium to a boil to dissolve the agar and autoclave (121°C for 15 min). Dry plates at room temperature for 2 - 3 days.<p> <b>Top layer:</b> Grow prey bacterium on agar slants using DSMZ medium 54 or medium 1. Prepare 5 ml aliquots of 1 % Bacto agar in 25 mM HEPES buffer (6.0 g/l HEPES, 0.6 g/l MgCl2 x 6 H2O, 0.3 g/l CaCl2 x 2 H2O, pH 7.2 ) and autoclave in test tubes. After cooling to 45°C in a water bath add 0.5 ml cell suspension of the host organism, mix, and pour the top layer. For the top layer of one plate use one 24 h old agar slant culture and wash off grown cells in 0.5 ml sterile water.<p>
2. Inoculate the top layer of the double-layer plates the same day when prepared.

Target organisms

No target organisms are recorded.

Provenance