Overview
- External ontology
- mediadive.medium:1012b (source link unavailable) — BACTERIOVORAX MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: DSMZ
Composition
flowchart LR
CultureMech_000434["bacteriovorax_medium"]
ing0_HEPES_buffer["HEPES buffer"]
CultureMech_000434 --> ing0_HEPES_buffer
CHEBI_46756["CHEBI:46756"]
ing0_HEPES_buffer -.-> CHEBI_46756
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000434 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000434 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | HEPES buffer | 6 G_PER_L |
CHEBI:46756
HEPES
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.6 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Grow prey bacteria on agar plates using the medium indicated below. Use one plate of a 24-hour-old culture and wash off grown cells in 5 ml sterile water.
- Prepare HEPES buffer, adjust pH to 7.2, and autoclave. After cooling to room temperature add 5 ml cell suspension of the host organism to 50 ml HEPES buffer and mix. The prey medium suspension should contain approx. 10<sup>7</sup> to 10<sup>9</sup> cells. Inoculate the complete medium immediately after preparation and incubate the suspension with shaking until a decrease in turbidity is visible.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:13.108168Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1012b |
| 2026-03-13T06:18:55.256153Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000434 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.296772+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BACTERIOVORAX MEDIUM" to "bacteriovorax_medium" for consistent matching |
| 2026-06-05T07:40:50.381734+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |