Overview
- External ontology
- mediadive.medium:1013 (source link unavailable) — HALOBACTERIOVORAX MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.5, 'max': 8.0}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1013.pdf
Composition
flowchart LR
CultureMech_000435["halobacteriovorax_medium"]
ing0_NaCl["NaCl"]
CultureMech_000435 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000435 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000435 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_KCl["KCl"]
CultureMech_000435 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaBr["NaBr"]
CultureMech_000435 --> ing4_NaBr
CHEBI_63004["CHEBI:63004"]
ing4_NaBr -.-> CHEBI_63004
ing5_H3BO3["H3BO3"]
CultureMech_000435 --> ing5_H3BO3
CHEBI_33118["CHEBI:33118"]
ing5_H3BO3 -.-> CHEBI_33118
ing6_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_000435 --> ing6_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing6_SrCl2_x_6_H2O -.-> CHEBI_36385
ing7_Citric_acid["Citric acid"]
CultureMech_000435 --> ing7_Citric_acid
CHEBI_30769["CHEBI:30769"]
ing7_Citric_acid -.-> CHEBI_30769
ing8_KI["KI"]
CultureMech_000435 --> ing8_KI
CHEBI_8346["CHEBI:8346"]
ing8_KI -.-> CHEBI_8346
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000435 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000435 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_000435 --> ing11_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing11_MnSO4_x_H2O -.-> CHEBI_86364
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000435 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_000435 --> ing13_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing13_CoSO4_x_7_H2O -.-> CHEBI_91244
ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000435 --> ing14_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000435 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_000435 --> ing16_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing16_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000435 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000435 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_000435 --> ing19_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing19_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000435 --> ing20_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing21_Bacto_peptone["Bacto peptone"]
CultureMech_000435 --> ing21_Bacto_peptone
ing22_Yeast_extract["Yeast extract"]
CultureMech_000435 --> ing22_Yeast_extract
ing23_Fe_III__citrate["Fe(III) citrate"]
CultureMech_000435 --> ing23_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing23_Fe_III__citrate -.-> CHEBI_144421
ing24_MgCl2["MgCl2"]
CultureMech_000435 --> ing24_MgCl2
CHEBI_6636["CHEBI:6636"]
ing24_MgCl2 -.-> CHEBI_6636
ing25_Na2SO4["Na2SO4"]
CultureMech_000435 --> ing25_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing25_Na2SO4 -.-> CHEBI_32149
ing26_CaCl2["CaCl2"]
CultureMech_000435 --> ing26_CaCl2
CHEBI_3312["CHEBI:3312"]
ing26_CaCl2 -.-> CHEBI_3312
ing27_NaHCO3["NaHCO3"]
CultureMech_000435 --> ing27_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing27_NaHCO3 -.-> CHEBI_32139
ing28_KBr["KBr"]
CultureMech_000435 --> ing28_KBr
CHEBI_32030["CHEBI:32030"]
ing28_KBr -.-> CHEBI_32030
ing29_SrCl2["SrCl2"]
CultureMech_000435 --> ing29_SrCl2
CHEBI_36383["CHEBI:36383"]
ing29_SrCl2 -.-> CHEBI_36383
CultureMech_000435 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
48.15 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 3 duplicates: 27.7, 1.0, 19.45] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 7.0, 3.0] |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
1.2000000000000002 G_PER_L |
CHEBI:32588
potassium chloride
|
[Merged 2 duplicates: 0.65, 0.55] |
| 5 |
NaBr
Br.Na 102.894 g/mol
|
0.1 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 6 |
H3BO3
H3BO3 61.833 g/mol
|
0.062 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 3 duplicates: 0.03, 0.01, 0.022] |
| 7 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.015 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 8 |
Citric acid
C6H8O7 192.123 g/mol
|
0.01 G_PER_L |
CHEBI:30769
citric acid
|
|
| 9 |
KI
I.K 166.002 g/mol
|
5e-05 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
2.35 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 2.25, 0.1] |
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 12 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.5 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 15 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 17 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 21 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 22 | Bacto peptone | 5 G_PER_L | ||
| 23 | Yeast extract | 1 G_PER_L | ||
| 24 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
0.1 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 25 |
MgCl2
2Cl.Mg 95.211 g/mol
|
5.9 G_PER_L |
CHEBI:6636
magnesium dichloride
|
|
| 26 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.24 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 27 |
CaCl2
Ca.2Cl 110.984 g/mol
|
1.8 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 28 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 29 |
KBr
Br.K 119.002 g/mol
|
0.08 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 30 |
SrCl2
2Cl.Sr 158.526 g/mol
|
0.034 G_PER_L |
CHEBI:36383
strontium dichloride
|
|
| 31 |
Na-silicate
2Na.O3Si 122.063 g/mol
|
0.004 G_PER_L |
CHEBI:60720
sodium silicate
|
|
| 32 |
NaF
F.Na 41.988 g/mol
|
0.0024 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 33 |
(NH4)NO3
H4N.NO3 80.043 g/mol
|
0.0016 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 34 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.008 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Preparation
- Mix ingredients and adjust pH of the final solution to 7.5 - 8.0. Sterilize by autoclaving at 121°C for 20 min. Grow prey bacterium (DSM 15416) on agar plates using medium 514. For 50 ml prey medium suspension use one agar plate incubated for 24 hours and wash off grown cells in 3 - 5 ml medium. The prey medium suspension should contain approx. 10<sup>7</sup> to 10<sup>9</sup> cells. Inoculate the complete medium immediately after preparation and incubate the suspension with shaking until a decrease in turbidity is visible.
- First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
- Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.
- Note: M.514 refers to Bacto Marine Broth Difco 2216 (BD 279110). Other brands (even if they refer to Difco2216) have not been tested and might not support growth for some strains.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.109774Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1013 |
| 2026-03-13T03:09:27.125822Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:18:55.268165Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000435 |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.312235+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOBACTERIOVORAX MEDIUM" to "halobacteriovorax_medium" for consistent matching |
| 2026-05-20T06:01:21.919044+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.5-8' -> {'min': 7.5, 'max': 8.0} |
| 2026-06-05T07:41:07.584021+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.457485+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |