Overview
- External ontology
- mediadive.medium:1021 (source link unavailable) — SODALIS GLOSSINIDIUS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1021.pdf Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The false LB constituent expansion is withdrawn. Each source's actual hydrolysate and preparation scope is retained. Existing unrelated chemical annotations and history are preserved, not recertified; no taxa-media growth association is added.
Ingredients (0)
No ingredients recorded.
Preparation
- Prepare Solution A according to the linked DSMZ 1021 protocol and adjust Solution A to pH 8.0. This is not a measured final-medium pH.
- For the solid preparation, sterilize Solution A and Solution B separately at 121 C for 20 min. Do not autoclave the serum-supplemented mixture.
- Cool the separately sterilized solutions to 50 C, then combine A and B.
- To 4 parts of the combined basal preparation, add 1 part sterile foetal or newborn calf serum. Preserve the reported ratio; no fixed serum batch dose or mass concentration is asserted.
Solutions
Combined basal preparation (Solutions A and B)
Combine the separately prepared A and B before serum supplementation. The source then specifies 4 parts base to 1 part serum; no fixed receiving-medium dose or final total volume is asserted.
Solution A
Dose: 0.9 L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 6.88889 G_PER_L | Source batch input: 6.2 g in Solution A. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| Lactalbumin hydrolysate | 9 G_PER_L | Source batch input: 8.1 g in Solution A. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| D-Glucose | 5.55556 G_PER_L | Source batch input: 5 g in Solution A. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| KCl | 0.277778 G_PER_L | Source batch input: 0.25 g in Solution A. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| MgCl2 x 6 H2O | 0.133333 G_PER_L | Source batch input: 0.12 g in Solution A. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| CaCl2 x 2 H2O | 0.277778 G_PER_L | Source batch input: 0.25 g in Solution A. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| NaCl | 9.66667 G_PER_L | Source batch input: 8.7 g in Solution A. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| NaHCO3 | 0.166667 G_PER_L | Source batch input: 0.15 g in Solution A. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| Agar | 18.8889 G_PER_L | Source batch input: 17 g in Solution A. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| Distilled water | 0.9 L | Source batch input: 900 ml in Solution A. Water is a stock batch input, not a final-volume marker. |
Provider-reported addition to the combined basal preparation before serum; not a final-medium concentration.
Solution B
Dose: 0.1 L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaH2PO4 x 2 H2O | 2.8 G_PER_L | Source batch input: 0.28 g in Solution B. The attached g/L value is the API-reported stock rate, not a final-medium concentration; original water input alone does not establish a measured final stock volume. |
| Distilled water | 0.1 L | Source batch input: 100 ml in Solution B. Water is a stock batch input, not a final-volume marker. |
Provider-reported addition to the combined basal preparation before serum; not a final-medium concentration.
Sterile foetal or newborn calf serum
Add 1 part to 4 parts combined basal preparation after cooling. The source permits either serum; no mass concentration, fixed dose or numeric percent basis is inferred.
Target organisms
No target organisms are recorded.
Recipe variants
- Name
- Liquid preparation described by DSMZ 1021
- Relationship
- PHYSICAL_STATE_VARIANT
- Description
- The source describes an alternative liquid preparation but does not provide a separate complete ingredient table.
- Modifications
- Filter sterilize the liquid preparation instead of autoclaving. No membrane pore size is specified.
- The source does not explicitly quantify the agar change for this alternative; no numeric agar amount is invented.
- Evidence
- Reference
- https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1021.pdf
- Supports
- SUPPORT
- Snippet
- Sterilize solution A and B separately at 121C for 20 min. Cool to 50C, then combine. To 4 parts of the above, add 1 part sterile foetal or new borne calf serum. Liquid medium should be filter sterilized instead of autoclaved.
- Explanation
- variants[0].modifications: Preserve the source's conditional liquid preparation, not an invented fully quantified broth recipe. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-08T23:33:46.995198+00:00; capture SHA256=1326bfc6d79f8ed3ad765909aa48d3e4efbb378516ada4a4087710860d8b7cc8.
- Name
- Casein hydrolysate alternative
- Relationship
- SUBSTITUTED_COMPONENT_VARIANT
- Description
- The original DSMZ recipe allows casein hydrolysate in place of lactalbumin hydrolysate.
- Modifications
- Replace the 8.10 g lactalbumin hydrolysate batch input in Solution A with 8.10 g casein hydrolysate, not both together. Other preparation instructions are unchanged.
- Evidence
- Reference
- https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1021.pdf
- Supports
- SUPPORT
- Snippet
- Lactalbumin hydrolysate or Casein hydrolysate 8.10 g
- Explanation
- variants[1].modifications: Preserve the source-explicit alternative omitted from the API, as a separate substitution rather than an additional ingredient. No unstated density, final volume, percent basis or chemical identifier is inferred. Locator=original recipe table/text; accessed=2026-10-08T23:33:46.995198+00:00; capture SHA256=1326bfc6d79f8ed3ad765909aa48d3e4efbb378516ada4a4087710860d8b7cc8.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.129815Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1021 |
| 2026-03-13T06:18:55.314298Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000443 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.364563+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SODALIS GLOSSINIDIUS MEDIUM" to "sodalis_glossinidius_medium" for consistent matching |
| 2026-06-05T07:41:45.986427+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-08T23:50:40.246918+00:00 | codex | RESTORED_SOURCE_SPECIFIC_HYDROLYSATE_FORMULATIONS_WITH_EVIDENCE | Restore source-specific hydrolysates, stock/basal/medium quantity boundaries, missing inputs and preparation; correct unsupported duplicate links. Preserve IDs and prior history. Unknown serum bases/doses and gas amounts remain unknown. |