Overview
- External ontology
- mediadive.medium:1023 (source link unavailable) — MANNING MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 2.5, 'max': 2.7}
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1023.pdf
Composition
flowchart LR
CultureMech_000445["manning_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_000445 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KCl["KCl"]
CultureMech_000445 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_K2HPO4["K2HPO4"]
CultureMech_000445 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000445 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Ca_NO3_2["Ca(NO3)2"]
CultureMech_000445 --> ing4_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing4_Ca_NO3_2 -.-> CHEBI_64205
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000445 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_Yeast_extract["Yeast extract"]
CultureMech_000445 --> ing6_Yeast_extract
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
6 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
0.02 G_PER_L |
CHEBI:64205
calcium nitrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
33.4 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 | Yeast extract | 0.2 G_PER_L |
Preparation
- Solution A is separately autoclaved and added to the medium. The medium is supplemented with Solution B (yeast extract). Medium pH adjusted by 0.1 N H2SO4 to pH 2.5 - 2.7.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.136616Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1023 |
| 2026-02-06T05:10:21.197404Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:18:55.336138Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000445 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.386938+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MANNING MEDIUM" to "manning_medium" for consistent matching |
| 2026-05-20T06:01:25.323861+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '2.5-2.7' -> {'min': 2.5, 'max': 2.7} |
| 2026-06-05T07:41:12.669906+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:40.652017+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:00.832440+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |