smithella_medium

Overview

External ontology

mediadive.medium:1030 (source link unavailable) — SMITHELLA MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1030.pdf

Composition

flowchart LR
    CultureMech_000452["smithella_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_000452 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000452 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000452 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_000452 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_000452 --> ing4_Yeast_extract
    ing5_Trypticase_peptone["Trypticase peptone"]
    CultureMech_000452 --> ing5_Trypticase_peptone
    ing6_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000452 --> ing6_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Sodium_resazurin -.-> CHEBI_8806
    ing7_Na2CO3["Na2CO3"]
    CultureMech_000452 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_2_Mercaptoethanesulfonate["2-Mercaptoethanesulfonate"]
    CultureMech_000452 --> ing8_2_Mercaptoethanesulfonate
    CHEBI_17905["CHEBI:17905"]
    ing8_2_Mercaptoethanesulfonate -.-> CHEBI_17905
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000452 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000452 --> ing10_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing11_NaCl["NaCl"]
    CultureMech_000452 --> ing11_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing11_NaCl -.-> CHEBI_26710
    ing12_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000452 --> ing12_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing12_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing13_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000452 --> ing13_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing13_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing14_H3BO3["H3BO3"]
    CultureMech_000452 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000452 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_000452 --> ing16__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing16__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000452 --> ing17_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing18_Na2SeO4["Na2SeO4"]
    CultureMech_000452 --> ing18_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing18_Na2SeO4 -.-> CHEBI_77775
    ing19_Crotonic_acid["Crotonic acid"]
    CultureMech_000452 --> ing19_Crotonic_acid
    CHEBI_41131["CHEBI:41131"]
    ing19_Crotonic_acid -.-> CHEBI_41131
    ing20_NaOH["NaOH"]
    CultureMech_000452 --> ing20_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing20_NaOH -.-> CHEBI_32145
    ing21_Biotin["Biotin"]
    CultureMech_000452 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_000452 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000452 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Riboflavin["Riboflavin"]
    CultureMech_000452 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000452 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000452 --> ing26_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_000452 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000452 --> ing28_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing29__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000452 --> ing29__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29__DL__alpha_Lipoic_acid -.-> CHEBI_16494

Ingredients (30)

Preparation

1. Dissolve ingredients (except carbonate, crotonate, coenzyme M, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into Hungate-type tubes or serum vials (e.g., 5 ml medium in Balch-type tubes) and autoclave. Add crotonate, coenzyme M, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of crotonate, coenzyme M and vitamins should be sterilized by filtration. Adjust pH of the complete medium to 7.0 - 7.2.
2. Note: Use 10% (v/v) as inoculum.
3. First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.
4. Dissolve crotonic acid in 800 ml distilled water, add around 100 ml of 10 N NaOH and adjust pH to around 7. Then add water to reach a volume of 1000 ml. Sterilize by filtration under 100% N2 gas atmosphere.

Solutions

Target organisms

No target organisms are recorded.

Provenance