medium_for_methylobacterium_podarium

CultureMech:000455 bacterial DEFINED SOLID_AGAR
Original name: MEDIUM FOR METHYLOBACTERIUM PODARIUM

Overview

External ontology
mediadive.medium:1032 (source link unavailable) — MEDIUM FOR METHYLOBACTERIUM PODARIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.3, 'max': 7.4}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1032.pdf

Composition

flowchart LR
    CultureMech_000455["medium_for_methylobacterium_podarium"]
    ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_000455 --> ing0_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
    ing1_KH2PO4["KH2PO4"]
    CultureMech_000455 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000455 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000455 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Methylamine["Methylamine"]
    CultureMech_000455 --> ing4_Methylamine
    CHEBI_16830["CHEBI:16830"]
    ing4_Methylamine -.-> CHEBI_16830
    ing5_Agar["Agar"]
    CultureMech_000455 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_EDTA["EDTA"]
    CultureMech_000455 --> ing6_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing6_EDTA -.-> CHEBI_4735
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000455 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000455 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9__NH4_6Mo7O24["(NH4)6Mo7O24"]
    CultureMech_000455 --> ing9__NH4_6Mo7O24
    CHEBI_91249["CHEBI:91249"]
    ing9__NH4_6Mo7O24 -.-> CHEBI_91249
    ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000455 --> ing10_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing10_CuSO4_x_5_H2O -.-> CHEBI_31440

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
7.9 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate
2 KH2PO4
H2O4P.K 136.084 g/mol
1.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.8 G_PER_L CHEBI:31206
ammonium chloride
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 Methylamine
CH5N 31.058 g/mol
1.4 G_PER_L CHEBI:16830
methylamine
6 Agar 15 G_PER_L CHEBI:2509
agar
7 EDTA
C10H16N2O8 292.244 g/mol
50 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
7.34 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.5 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
10 (NH4)6Mo7O24
2H4N.MoO4 196.014 g/mol
0.5 G_PER_L CHEBI:91249
ammonium molybdate
11 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate

Preparation

  1. initial pH 7.3 - 7.4
  2. sterilize by 115°C / 10 min
  3. Dissolve the EDTA in about 400 ml of water, then add 9 g NaOH. Dissolve each of the salts individually in about 40-50 ml of water and add them to the EDTA-NaOH solution. Adjust the final pH of the solution to pH 6.0 with 1M NaOH (about 24 ml). Make up to 1 litre with distilled water, store in the dark and do not autoclave the stock solution before it is added to the medium.

Solutions

Trace elements solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
ZnSO4 x 7 H2O 1 G_PER_L
MnCl2 x 4 H2O 2.5 G_PER_L
FeSO4 x 7 H2O 5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.161847Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1032
2026-03-13T06:18:55.462321Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000455
2026-03-17T06:29:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.473636+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR METHYLOBACTERIUM PODARIUM" to "medium_for_methylobacterium_podarium" for consistent matching
2026-05-20T06:01:54.080051+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.3-7.4' -> {'min': 7.3, 'max': 7.4}
2026-06-05T07:41:29.179138+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.087335+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.529977+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace elements solution' @ 10 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).