alkaliphilic_halomonas_medium (CultureMech:000457)

Overview

External ontology

mediadive.medium:1034 (source link unavailable) — ALKALIPHILIC HALOMONAS MEDIUM

Applications

Microbial cultivation

pH

9.0

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1034.pdf

Composition

flowchart LR
    CultureMech_000457["alkaliphilic_halomonas_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_000457 --> ing0_Yeast_extract
    ing1_Sodium_citrate["Sodium citrate"]
    CultureMech_000457 --> ing1_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing1_Sodium_citrate -.-> CHEBI_53258
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000457 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_Na2CO3["Na2CO3"]
    CultureMech_000457 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_NaCl["NaCl"]
    CultureMech_000457 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000457 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_000457 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836

Ingredients (7)

#	Ingredient	Concentration	Ontology
1	Yeast extract	10 G_PER_L
2	Sodium citrate C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate
3	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	1 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
4	Na2CO3 CO3.2Na 105.988 g/mol	3 G_PER_L	CHEBI:29377 sodium carbonate
5	NaCl Cl.Na 58.443 g/mol	100 G_PER_L	CHEBI:26710 sodium chloride
6	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.36 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate
7	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	50 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate

Preparation

Na2CO3 and NaCl were autoclaved separately.
Adjust to pH 9.0

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:13.167047Z	mediadive-import	Imported from MediaDive	Source: DSMZ, ID: 1034
2026-03-13T06:18:55.478260Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000457
2026-03-17T06:30:23.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.488973+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ALKALIPHILIC HALOMONAS MEDIUM" to "alkaliphilic_halomonas_medium" for consistent matching
2026-06-05T07:40:48.005135+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.838874+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.