Overview
- External ontology
- mediadive.medium:1038 (source link unavailable) — ACIDICALDUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 2.5
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1038.pdf
Composition
flowchart LR
CultureMech_000461["acidicaldus_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_000461 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KCl["KCl"]
CultureMech_000461 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_000461 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KH2PO4["KH2PO4"]
CultureMech_000461 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_000461 --> ing4_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing5_D_Glucose["D-Glucose"]
CultureMech_000461 --> ing5_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing5_D_Glucose -.-> CHEBI_17634
ing6_Yeast_extract["Yeast extract"]
CultureMech_000461 --> ing6_Yeast_extract
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.45 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.05 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.05 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.02 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 6 |
D-Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 7 | Yeast extract | 0.2 G_PER_L |
Preparation
- Dissolve ingredients except glucose and yeast extract, adjust pH to 2.5, dispense in suitable cultivation vessels and autoclave. Add glucose and yeast extract after autoclaving from sterile stock solutions.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.175200Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1038 |
| 2026-03-13T06:18:55.511192Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000461 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.514064+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDICALDUS MEDIUM" to "acidicaldus_medium" for consistent matching |
| 2026-06-05T07:40:47.166799+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.545709+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T13:32:40.086487+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |