desulfuromusa_ferrireducens_medium

Overview

External ontology

mediadive.medium:1041 (source link unavailable) — DESULFUROMUSA FERRIREDUCENS MEDIUM

Applications

• Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

Notes

Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1041.pdf

Composition

flowchart LR
    CultureMech_000464["desulfuromusa_ferrireducens_medium"]
    ing0_Sea_Salt["Sea Salt"]
    CultureMech_000464 --> ing0_Sea_Salt
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000464 --> ing1_Yeast_extract
    ing2_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000464 --> ing2_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Sodium_resazurin -.-> CHEBI_8806
    ing3_NaHCO3["NaHCO3"]
    CultureMech_000464 --> ing3_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing3_NaHCO3 -.-> CHEBI_32139
    ing4_Na_acetate["Na-acetate"]
    CultureMech_000464 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5_Na2_fumarate["Na2-fumarate"]
    CultureMech_000464 --> ing5_Na2_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing5_Na2_fumarate -.-> CHEBI_115156
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000464 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000464 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_NaCl["NaCl"]
    CultureMech_000464 --> ing8_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing8_NaCl -.-> CHEBI_26710
    ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000464 --> ing9_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000464 --> ing10_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing11_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_000464 --> ing11_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing11_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing12_H3BO3["H3BO3"]
    CultureMech_000464 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000464 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_000464 --> ing14__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing14__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing15_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_000464 --> ing15_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing15_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing16_Na2SeO4["Na2SeO4"]
    CultureMech_000464 --> ing16_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing16_Na2SeO4 -.-> CHEBI_77775
    ing17_Biotin["Biotin"]
    CultureMech_000464 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Folic_acid["Folic acid"]
    CultureMech_000464 --> ing18_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing18_Folic_acid -.-> CHEBI_27470
    ing19_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000464 --> ing19_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing19_Thiamine_HCl -.-> CHEBI_49105
    ing20_Riboflavin["Riboflavin"]
    CultureMech_000464 --> ing20_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing20_Riboflavin -.-> CHEBI_17015
    ing21_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000464 --> ing21_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing21_Nicotinic_acid -.-> CHEBI_15940
    ing22_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_000464 --> ing22_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing22_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing23_Vitamin_B12["Vitamin B12"]
    CultureMech_000464 --> ing23_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing23_Vitamin_B12 -.-> CHEBI_176843
    ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000464 --> ing24_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing25__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_000464 --> ing25__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing25__DL__alpha_Lipoic_acid -.-> CHEBI_16494

Ingredients (26)

Preparation

1. Dissolve ingredients (except bicarbonate, acetate, fumarate, vitamins, and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add acetate, fumarate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and fumarate should be sterilized by filtration. Adjust pH of the complete medium to 7.0-7.2, if necessary.
2. First adjust pH to 1.0 with diluted H2SO4, then add and dissolve the salts.

Solutions

Target organisms

No target organisms are recorded.

Provenance