Overview
- External ontology
- mediadive.medium:1047 (source link unavailable) — DIFCO RAKA-RAY NO.3 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.4
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1047.pdf
Composition
flowchart LR
CultureMech_000470["difco_raka_ray_no_3_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_000470 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_000470 --> ing1_Tryptone
ing2_Liver_Concentrate["Liver Concentrate"]
CultureMech_000470 --> ing2_Liver_Concentrate
ing3_Maltose["Maltose"]
CultureMech_000470 --> ing3_Maltose
CHEBI_17306["CHEBI:17306"]
ing3_Maltose -.-> CHEBI_17306
ing4_Fructose["Fructose"]
CultureMech_000470 --> ing4_Fructose
CHEBI_28757["CHEBI:28757"]
ing4_Fructose -.-> CHEBI_28757
ing5_Glucose["Glucose"]
CultureMech_000470 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Betaine_Hydrochloride["Betaine Hydrochloride"]
CultureMech_000470 --> ing6_Betaine_Hydrochloride
ing7_Diammonium_Citrate["Diammonium Citrate"]
CultureMech_000470 --> ing7_Diammonium_Citrate
CHEBI_63076["CHEBI:63076"]
ing7_Diammonium_Citrate -.-> CHEBI_63076
ing8_Potassium_Aspartate["Potassium Aspartate"]
CultureMech_000470 --> ing8_Potassium_Aspartate
ing9_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_000470 --> ing9_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing9_Magnesium_sulfate -.-> CHEBI_32599
ing10_Manganese_Sulfate["Manganese Sulfate"]
CultureMech_000470 --> ing10_Manganese_Sulfate
CHEBI_86364["CHEBI:86364"]
ing10_Manganese_Sulfate -.-> CHEBI_86364
ing11_Dipotassium_Phosphate["Dipotassium Phosphate"]
CultureMech_000470 --> ing11_Dipotassium_Phosphate
CHEBI_131527["CHEBI:131527"]
ing11_Dipotassium_Phosphate -.-> CHEBI_131527
ing12_N_Acetylglucosamine["N-Acetylglucosamine"]
CultureMech_000470 --> ing12_N_Acetylglucosamine
CHEBI_59640["CHEBI:59640"]
ing12_N_Acetylglucosamine -.-> CHEBI_59640
ing13_Potassium_Glutamate["Potassium Glutamate"]
CultureMech_000470 --> ing13_Potassium_Glutamate
ing14_Agar["Agar"]
CultureMech_000470 --> ing14_Agar
CHEBI_2509["CHEBI:2509"]
ing14_Agar -.-> CHEBI_2509
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | ||
| 2 | Tryptone | 20 G_PER_L | ||
| 3 | Liver Concentrate | 1 G_PER_L | ||
| 4 |
Maltose
C12H22O11 342.297 g/mol
|
10 G_PER_L |
CHEBI:17306
maltose
|
|
| 5 | Fructose | 5 G_PER_L |
CHEBI:28757
fructose
|
|
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 7 | Betaine Hydrochloride | 2 G_PER_L | ||
| 8 |
Diammonium Citrate
C6H6O7.2H4N 226.185 g/mol
|
2 G_PER_L |
CHEBI:63076
diammonium citrate
|
|
| 9 | Potassium Aspartate | 2.5 G_PER_L | ||
| 10 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.98 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 11 |
Manganese Sulfate
H2O.Mn.O4S 169.016 g/mol
|
0.42 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 12 |
Dipotassium Phosphate
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 13 | N-Acetylglucosamine | 0.5 G_PER_L |
CHEBI:59640
N-acetylglucosamine
|
|
| 14 | Potassium Glutamate | 2.5 G_PER_L | ||
| 15 | Agar | 16 G_PER_L |
CHEBI:2509
agar
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.199753Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1047 |
| 2026-03-13T06:18:55.588466Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000470 |
| 2026-03-17T06:29:50.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.593064+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DIFCO RAKA-RAY NO.3 MEDIUM" to "difco_raka_ray_no_3_medium" for consistent matching |
| 2026-06-05T07:40:58.713074+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |