selenate_reducer_medium

CultureMech:000472 bacterial DEFINED SOLID_AGAR
Original name: SELENATE REDUCER MEDIUM

Overview

External ontology
mediadive.medium:1049 (source link unavailable) — SELENATE REDUCER MEDIUM
Applications
  • Microbial cultivation
pH
7.0
High-metal flag
True
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1049.pdf

Composition

flowchart LR
    CultureMech_000472["selenate_reducer_medium"]
    ing0_DL_Na_lactate["DL-Na-lactate"]
    CultureMech_000472 --> ing0_DL_Na_lactate
    CHEBI_75228["CHEBI:75228"]
    ing0_DL_Na_lactate -.-> CHEBI_75228
    ing1_KCl["KCl"]
    CultureMech_000472 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_KH2PO4["KH2PO4"]
    CultureMech_000472 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_NaCl["NaCl"]
    CultureMech_000472 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_NH4Cl["NH4Cl"]
    CultureMech_000472 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000472 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000472 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_Resazurin["Resazurin"]
    CultureMech_000472 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Agar["Agar"]
    CultureMech_000472 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Na2SeO4["Na2SeO4"]
    CultureMech_000472 --> ing9_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing9_Na2SeO4 -.-> CHEBI_77775
    ing10_NaHCO3["NaHCO3"]
    CultureMech_000472 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_000472 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Na2SeO3["Na2SeO3"]
    CultureMech_000472 --> ing12_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing12_Na2SeO3 -.-> CHEBI_48843
    ing13_Na2WO4["Na2WO4"]
    CultureMech_000472 --> ing13_Na2WO4
    CHEBI_63940["CHEBI:63940"]
    ing13_Na2WO4 -.-> CHEBI_63940
    ing14_1_4_Naphthoquinone["1,4-Naphthoquinone"]
    CultureMech_000472 --> ing14_1_4_Naphthoquinone
    CHEBI_27418["CHEBI:27418"]
    ing14_1_4_Naphthoquinone -.-> CHEBI_27418
    ing15_Hemin["Hemin"]
    CultureMech_000472 --> ing15_Hemin
    CHEBI_50385["CHEBI:50385"]
    ing15_Hemin -.-> CHEBI_50385
    ing16_HCl["HCl"]
    CultureMech_000472 --> ing16_HCl
    CHEBI_17883["CHEBI:17883"]
    ing16_HCl -.-> CHEBI_17883
    ing17_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_000472 --> ing17_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing17_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing18_ZnCl2["ZnCl2"]
    CultureMech_000472 --> ing18_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing18_ZnCl2 -.-> CHEBI_49976
    ing19_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000472 --> ing19_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing19_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing20_H3BO3["H3BO3"]
    CultureMech_000472 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000472 --> ing21_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing21_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing22_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000472 --> ing22_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing22_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing23_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000472 --> ing23_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing23_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000472 --> ing24_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing25_Vitamin_B12["Vitamin B12"]
    CultureMech_000472 --> ing25_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing25_Vitamin_B12 -.-> CHEBI_176843
    ing26_Pantothenic_acid["Pantothenic acid"]
    CultureMech_000472 --> ing26_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing26_Pantothenic_acid -.-> CHEBI_7916
    ing27_Riboflavin["Riboflavin"]
    CultureMech_000472 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_000472 --> ing28_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing28_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing29_Biotin["Biotin"]
    CultureMech_000472 --> ing29_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing29_Biotin -.-> CHEBI_15956
    CultureMech_000472 --> more["...6 more"]

Ingredients (36)

# Ingredient Concentration Ontology Notes
1 DL-Na-lactate
C3H5O3.Na 112.06 g/mol
1.19149 G_PER_L CHEBI:75228
sodium lactate
2 KCl
Cl.K 74.551 g/mol
1.38298 G_PER_L CHEBI:32588
potassium chloride
3 KH2PO4
H2O4P.K 136.084 g/mol
0.212766 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 NaCl
Cl.Na 58.443 g/mol
24.4681 G_PER_L CHEBI:26710
sodium chloride
5 NH4Cl
Cl.H4N 53.492 g/mol
0.531915 G_PER_L CHEBI:31206
ammonium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.106383 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
3.19149 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 Resazurin
C12H7NO4 229.191 g/mol
0.000531915 G_PER_L CHEBI:8806
Resazurin
9 Agar 15.9574 G_PER_L CHEBI:2509
agar
10 Na2SeO4
2Na.O4Se 188.936 g/mol
189 G_PER_L CHEBI:77775
sodium selenate
11 NaHCO3
CHO3.Na 84.006 g/mol
100 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Na2SeO3
2Na.O3Se 172.937 g/mol
0.03 G_PER_L CHEBI:48843
disodium selenite
14 Na2WO4
2Na.O4W 293.816 g/mol
0.08 G_PER_L CHEBI:63940
sodium tungstate
15 1,4-Naphthoquinone
C10H6O2 158.156 g/mol
0.02 G_PER_L CHEBI:27418
1,4-naphthoquinone
16 Hemin
C34H32ClFeN4O4 651.952 g/mol
0.005 G_PER_L CHEBI:50385
hemin
17 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
18 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
19 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
20 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
21 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
22 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
23 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
24 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
25 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
26 Vitamin B12 0.05 G_PER_L CHEBI:176843
vitamin B12
27 Pantothenic acid
C9H17NO5 219.237 g/mol
0.05 G_PER_L CHEBI:7916
pantothenic acid
28 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
29 Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
0.01 G_PER_L CHEBI:131531
pyridoxamine hydrochloride
30 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
31 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
32 Nicotinic acid
C6H5NO2 123.111 g/mol
0.025 G_PER_L CHEBI:15940
nicotinic acid
33 Nicotine amide
C6H6N2O 122.127 g/mol
0.025 G_PER_L CHEBI:17154
nicotinamide
34 alpha-lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
35 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.05 G_PER_L CHEBI:30753
4-aminobenzoic acid
36 Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
0.05 G_PER_L CHEBI:132751
thiamine hydrochloride dihydrate

Preparation

  1. After cooling of medium A, add per 100 ml: 1.0 ml solution B, 2.5 ml solution C, 1.0 ml solution D, 0.1 ml solution E, 0.5 ml solution F, 1.0 ml solution G and 1.0 ml solution H.
  2. Adjust pH to 7.0.
  3. It is recommended to cultivate strain DSM 17993 on agar plates which may be prepared under aerobic conditions. Plates are then preincubated under anaerobic conditions prior to inoculation. They will stay pink coloured.
  4. For liquid medium, prepare solution A, distribute to anaerobic cultivation vessels, heat to the boil and cool to room temperature while gassing with N2 : CO2 (80:20). Close vessels and autoclave. Prepare solutions B, C, D, E and H under anaerobic conditions and autoclave. Solutions F and H are filter sterilized and flushed with N2. Selenate (solution B) might be replaced by nitrate (solution: 1.00 g KNO3 in 10 ml).
  5. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
  6. Stir for some hours, filter sterilize the solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.203685Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1049
2026-03-13T06:18:55.607331Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000472
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.612100+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SELENATE REDUCER MEDIUM" to "selenate_reducer_medium" for consistent matching
2026-06-05T07:41:45.654468+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.828602+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:11.419694+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.