py_x_medium

CultureMech:000475 bacterial COMPLEX LIQUID
Original name: PY + X MEDIUM

Overview

External ontology
mediadive.medium:104b (source link unavailable) — PY + X MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 6.8, 'max': 7.0}
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf

Composition

flowchart LR
    CultureMech_000475["py_x_medium"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_000475 --> ing0_Trypticase_peptone
    ing1_Meat_peptone["Meat peptone"]
    CultureMech_000475 --> ing1_Meat_peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_000475 --> ing2_Yeast_extract
    ing3_Sodium_resazurin["Sodium resazurin"]
    CultureMech_000475 --> ing3_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Sodium_resazurin -.-> CHEBI_8806
    ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_000475 --> ing4_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing5_D_Glucose["D-Glucose"]
    CultureMech_000475 --> ing5_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing5_D_Glucose -.-> CHEBI_17634
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000475 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000475 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_K2HPO4["K2HPO4"]
    CultureMech_000475 --> ing8_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing8_K2HPO4 -.-> CHEBI_131527
    ing9_KH2PO4["KH2PO4"]
    CultureMech_000475 --> ing9_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing9_KH2PO4 -.-> CHEBI_63036
    ing10_NaHCO3["NaHCO3"]
    CultureMech_000475 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_NaCl["NaCl"]
    CultureMech_000475 --> ing11_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing11_NaCl -.-> CHEBI_26710

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Trypticase peptone 5 G_PER_L
2 Meat peptone 5 G_PER_L
3 Yeast extract 10 G_PER_L
4 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
5 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
6 D-Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17634
D-glucose
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
10 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
11 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 NaCl
Cl.Na 58.443 g/mol
2 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. Dissolve ingredients (except cysteine and glucose) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 7.0, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose or any other substrate from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.8 - 7.0, if necessary.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:10.174109Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 104b
2026-03-13T06:18:55.633375Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000475
2026-03-17T06:29:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.639931+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PY + X MEDIUM" to "py_x_medium" for consistent matching
2026-05-20T06:02:03.243472+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '6.8-7' -> {'min': 6.8, 'max': 7.0}
2026-06-05T07:41:42.958720+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:10.557491+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.