Overview
- External ontology
- mediadive.medium:1057 (source link unavailable) — ETHANOLIGENES MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1057.pdf
Composition
flowchart LR
CultureMech_000486["ethanoligenes_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_000486 --> ing0_Tryptone
ing1_Beef_extract["Beef extract"]
CultureMech_000486 --> ing1_Beef_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_000486 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_000486 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_K2HPO4["K2HPO4"]
CultureMech_000486 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_000486 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000486 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_000486 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_000486 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_D_Glucose["D-Glucose"]
CultureMech_000486 --> ing9_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing9_D_Glucose -.-> CHEBI_17634
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_000486 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_000486 --> ing11_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing11_FeCl2_x_4_H2O -.-> CHEBI_86249
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000486 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000486 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000486 --> ing14_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
ing15_ZnCl2["ZnCl2"]
CultureMech_000486 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_CuCl2["CuCl2"]
CultureMech_000486 --> ing16_CuCl2
CHEBI_49553["CHEBI:49553"]
ing16_CuCl2 -.-> CHEBI_49553
ing17_H3BO3["H3BO3"]
CultureMech_000486 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000486 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_000486 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_000486 --> ing20_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_000486 --> ing21_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing22_Biotin["Biotin"]
CultureMech_000486 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_000486 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_000486 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Thiamine_HCl["Thiamine HCl"]
CultureMech_000486 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Riboflavin["Riboflavin"]
CultureMech_000486 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_000486 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_000486 --> ing28_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_D_____pantothenate -.-> CHEBI_31345
ing29_Vitamin_B12["Vitamin B12"]
CultureMech_000486 --> ing29_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing29_Vitamin_B12 -.-> CHEBI_176843
CultureMech_000486 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 3.99202 G_PER_L | ||
| 2 | Beef extract | 1.99601 G_PER_L | ||
| 3 | Yeast extract | 0.998004 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
4.99202 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 3.99202, 1.0] |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1.49701 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0998004 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.199601 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000499002 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.499002 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
D-Glucose
C6H12O6 180.156 g/mol
|
9.98004 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 12 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.03 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 17 |
CuCl2
Cl2Cu 134.452 g/mol
|
0.02 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.1 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.03 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 22 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.04 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 26 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 29 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 | Vitamin B12 | 0.001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 31 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 32 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients (except cysteine, glucose and vitamins), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 6.0, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add glucose and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Adjust pH of complete medium to 6.0, if necessary.
- First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. Finally adjust pH to 6.5 with KOH and make up to 1000.00 ml.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:13.237456Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 1057 |
| 2026-02-06T05:10:21.855974Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:53.096940Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:18:55.753986Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000486 |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.762530+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ETHANOLIGENES MEDIUM" to "ethanoligenes_medium" for consistent matching |
| 2026-06-05T07:40:59.672004+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.730820+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |