halothiobacillus_hl2_medium

CultureMech:000487 bacterial DEFINED LIQUID
Original name: HALOTHIOBACILLUS HL2 MEDIUM

Overview

External ontology
mediadive.medium:1058b (source link unavailable) — HALOTHIOBACILLUS HL2 MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1058b.pdf

Composition

flowchart LR
    CultureMech_000487["halothiobacillus_hl2_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_000487 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000487 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_000487 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000487 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_000487 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_000487 --> ing5_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing6_NaHCO3["NaHCO3"]
    CultureMech_000487 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_EDTA["EDTA"]
    CultureMech_000487 --> ing7_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing7_EDTA -.-> CHEBI_4735
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000487 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000487 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_H3BO3["H3BO3"]
    CultureMech_000487 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000487 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing12_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_000487 --> ing12_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing12_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_000487 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000487 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_000487 --> ing15_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing16_D_____biotin["D-(+)-biotin"]
    CultureMech_000487 --> ing16_D_____biotin
    CHEBI_15956["CHEBI:15956"]
    ing16_D_____biotin -.-> CHEBI_15956
    ing17_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000487 --> ing17_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
119.76 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
1.49701 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.499002 G_PER_L CHEBI:31206
ammonium chloride
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0499002 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.399202 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
4.99002 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
7 NaHCO3
CHO3.Na 84.006 g/mol
2.49501 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
13 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.03 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
17 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
18 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Dissolve sodium chloride, potassium hydrogenphosphate and ammonium chloride, then sparge solution with 80% N2 and 20% CO2 gas mixture for at least 30 - 45 min to remove dissolved oxygen and to saturate the solution with CO2. Dispense solution under air atmosphere in Erlenmeyer flasks to 10% of volume, close with screw caps and autoclave. Add trace elements, calcium chloride, magnesium chloride, thiosulfate, bicarbonate and vitamins from sterile stock solutions and adjust pH of the medium to 7.5 using a sterile stock solution of sodium carbonate (5% w/v). Incubate with shaking.
  2. Note: Use at least 10% (v/v) as inoculum.
  3. pH 3.0-4.0

Solutions

Trace elements solution (Pfennig, 1965)

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 2.2 G_PER_L

Seven vitamins solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.1 G_PER_L
Nicotinic acid 0.2 G_PER_L
Pyridoxine hydrochloride 0.3 G_PER_L
Thiamine-HCl x 2 H2O 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:13.249215Z mediadive-import Imported from MediaDive Source: DSMZ, ID: 1058b
2026-03-13T06:18:55.766275Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000487
2026-03-17T06:30:32.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:06.776125+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALOTHIOBACILLUS HL2 MEDIUM" to "halothiobacillus_hl2_medium" for consistent matching
2026-06-05T07:41:08.254343+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.683012+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T07:05:22.308928+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace elements solution (Pfennig, 1965)' @ 1 ml/l; 4 -> 'Seven vitamins solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).