Overview
- External ontology
- mediadive.medium:105 (source link unavailable) — GLUCONOBACTER OXYDANS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: DSMZ | Link: https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium105.pdf
Composition
flowchart LR
CultureMech_000492["gluconobacter_oxydans_medium"]
ing0_Glucose["Glucose"]
CultureMech_000492 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_Yeast_extract["Yeast extract"]
CultureMech_000492 --> ing1_Yeast_extract
ing2_CaCO3["CaCO3"]
CultureMech_000492 --> ing2_CaCO3
CHEBI_3311["CHEBI:3311"]
ing2_CaCO3 -.-> CHEBI_3311
ing3_Agar["Agar"]
CultureMech_000492 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
100 G_PER_L |
CHEBI:17234
glucose
|
|
| 2 | Yeast extract | 10 G_PER_L | ||
| 3 |
CaCO3
CO3.Ca 100.086 g/mol
|
20 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Preparation
- Adjust pH to 6.8.
- Autoclave at 121 °C for 15 min.
- Note: 20 g calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. In liquid culture the calcium carbonate also forms a sediment that clears as acid is produced.
Target organisms and growth evidence
These are curated observations and conditions for named organisms; they do not imply universal growth suitability.
- Preferred term
- GLUCONOBACTER OXYDANS
- Term
- Id
- NCBITaxon:442
- Label
- Gluconobacter oxydans
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:10.180153Z | mediadive-import | Imported from MediaDive | Source: DSMZ, ID: 105 |
| 2026-02-20T07:49:55.000000Z | organism-data-backfill | Added curated organism data | Added organism: GLUCONOBACTER OXYDANS, culture_type: isolate |
| 2026-03-13T06:18:55.819206Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000492 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:06.834725+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GLUCONOBACTER OXYDANS MEDIUM" to "gluconobacter_oxydans_medium" for consistent matching |
| 2026-06-05T07:41:06.389777+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T10:55:41.454416+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |